RNAi技术敲减乳腺癌MDA-MB-231细胞SIRT2基因的研究  

Studies on SIRT2 gene knockdown in breast cancer MDA-MB-231 cells by RNAi technique

作　　者：张舟[1] 邓小冲 章骏[1] 夏振华[1] 房林[2] Zhang Zhou;Deng Xiaochong;Zhang Jun(Dept of Surgery,Shidong Hospital,Yangpu Destrict,Shanghai 200438;Dept of General Surgery,Tenth People s Hospital of Tongji University,Shanghai 200072)

机构地区：[1]上海市杨浦区市东医院外二科,上海200438 [2]同济大学附属第十人民医院甲状腺乳腺外科,上海200072

出　　处：《安徽医科大学学报》2020年第2期245-249,共5页Acta Universitatis Medicinalis Anhui

基　　金：上海市卫生和计划生育委员会科研课题基金(编号:20164Y0276)。

摘　　要：目的研究去乙酰化蛋白2(SIRT2)对人乳腺癌MDA-MB-231细胞增殖、迁移能力的影响。方法采用实时荧光定量PCR(qRT-PCR)法检测SIRT2在乳腺癌MDA-MB-231细胞中mRNA的表达情况。使用小RNA干扰技术(siRNA)处理MDA-MB-231细胞,实验组转染靶向SIRT2的siRNA,对照组转染阴性对照序列,分别使用MTT比色法和平板细胞克隆形成实验检测MDA-MB-231细胞的增殖情况,使用细胞划痕实验检测MDA-MB-231细胞的迁移能力。结果转染SIRT2-siRNA的MDA-MB-231细胞SIRT2 mRNA表达低于阴性对照组(P<0.01);转染SIRT2-siRNA后,MDA-MB-231细胞的增殖能力、克隆形成能力以及迁移能力均减低(P<0.05)。结论敲减SIRT2后乳腺癌MDA-MB-231细胞增殖与迁移能力受到抑制,SIRT2在三阴性乳腺癌中起着促癌基因的作用。Objective To study the effect of sirtuin-2(SIRT2)on proliferation and migration of human breast cancer MDA-MB-231 cells.Methods Real-time fluorescence quantitative PCR(qRT-PCR)was applied to detect SIRT2 mRNA expression in breast cancer MDA-MB-231 cells.MDA-MB-231 cells were transfected with small interference RNA(siRNA),targeting SIRT2 in the experimental group,and also with negative control sequence in the control group.The proliferation ability of MDA-MB-231 cells was detected by MTT assay and cell colony formation assay,and the migration abilityof MDA-MB-231 cells was assessed through wound healing assay.Results The expression of SIRT2 mRNA in MDA-MB-231 cells transfected with SIRT2-siRNA was lower than that in the negative control group(P<0.01).After transfection with SIRT2-siRNA,cell proliferation,clonality and migration of MDA-MB-231 cells decreased(P<0.05).Conclusion The proliferation and migration of breast cancer MDA-MB-231 cells were inhibited after SIRT2 knockdown,which suggested that SIRT2 might function as an oncogene in triple negative breast cancer cells.

关 键 词：SIRT2 乳腺癌 MDA-MB-231细胞 增殖 迁移 

分 类 号：R737.9[医药卫生—肿瘤]