HPLC法同时测定采后莲雾果实木质素代谢途径中5种酚酸的含量  

Simultaneous Determination of 5 Phenolic Acids Involved in the Phenylpropanoid Pathway of Postharvest Wax Apple(Syzygium samarangenese[Blume]Merrill&L.M.Perry)by High Performance Liquid Chromatography

作　　者：匡凤元 吴光斌 张珅 黄利娜 陈发河 KUANG Fengyuan;WU Guangbin;ZHANG Shen;HUANG Lina;CHEN Fahe(College of Food and Biological Engineering,Jimei University,Xiamen 361000,China)

机构地区：[1]集美大学食品与生物工程学院,福建厦门361000

出　　处：《食品科学》2020年第8期165-170,共6页Food Science

基　　金：国家自然科学基金面上项目(31171777);福建省自然科学基金项目(2018J01448)。

摘　　要：为探讨采后莲雾果实贮藏期间酚酸含量变化对果实木质化代谢的影响,建立高效液相色谱法同时测定采后木质素代谢途径中5种酚酸(肉桂酸、对香豆酸、咖啡酸、阿魏酸、芥子酸)的方法。采用InfinityLab Poroahell C18色谱柱为固定相,以甲醇和乙酸溶液(pH 2.4)为流动相进行梯度洗脱,流速为0.8 mL/min,柱温25℃,紫外检测器于波长279 nm和320 nm处进行检测。各酚酸组分的线性范围较宽,相关系数均大于0.9994,检出限为0.01~0.03 mg/kg,定量限为0.03~0.09 mg/kg,相对标准偏差小于5%,加标回收率为86.18%~99.05%。该方法准确度、灵敏度高,操作简便,适用于采后莲雾果实木质素代谢途径中5种酚酸的同时测定。测得莲雾贮藏期间肉桂酸、对香豆酸、咖啡酸、阿魏酸和芥子酸的含量范围分别为11.46~24.69、0.92~1.45、2.31~5.32、8.86~20.58 mg/kg和9.48~22.62 mg/kg。A simple and sensitive high performance liquid chromatography(HPLC)method was established for simultaneous determination of 5 phenolic acids(trans-cinnamic acid,p-coumaric acid,caffeic acid,ferulic acid and sinapic acid)involved in the lignin synthesis of harvested wax apple.The chromatographic separation was achieved using an InfinityLab Poroahell C18 column through gradient elution with a mobile phases consisting of methanol and acetic acid aqueous solution(pH 2.4)at a flow rate of 0.8 mL/min.The column temperature was set at 25℃.The detection wavelengths were set at 279 and 320 nm.The method exhibited a wide linear range with correlation coefficients all above 0.9994.The limits of detection(LODs)were in the range of 0.01–0.03 mg/kg and the limits of quantitation(LOQs)were in the range of 0.03–0.09 mg/kg.The spiked recoveries ranged from 86.18%–99.05%,with relative standard deviations(RSDs)less than 5%.This method proved to be accurate,sensitive and simple and could be applied to determine the 5 phenolic acids in wax apple.The measured contents of trans-cinnamic acid,p-coumaric acid,caffeic acid,ferulic acid and sinapic acid were 11.46–24.69,0.92–1.45,2.31–5.32,8.86–20.58 and 9.48–22.62 mg/kg in wax apple,respectively.

关 键 词：高效液相色谱 莲雾 酚酸 木质素 测定方法 

分 类 号：O657.72[理学—分析化学]