基于醇溶蛋白的木兰科植物鉴定技术研究  

Study on the identification of Magnoliaceae plants based on the alcohol-soluble protein

作　　者：叶珊珊 王楠 高艳芳 唐密 刘梦龄 肖强 YE Shan-shan;WANG Nan;GAO Yan-fang;TANG Mi;LIU Meng-ling;XIAO Qiang(Hubei key Laboratory of Biological Resources Protection and Utilization,Hubei Minzu University,Enshi 445000,Hubei,China;School of Forestry and Horticulture,Hubei Minzu University,Enshi 445000,Hubei,China)

机构地区：[1]湖北民族大学生物资源保护与利用湖北省重点实验室,湖北恩施445000 [2]湖北民族大学林学园艺学院,湖北恩施445000

出　　处：《湖北农业科学》2020年第2期98-101,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31260057,31460203);湖北省大学生创新创业训练计划项目(201710517038,201810517051)。

摘　　要：木兰科(Magnoliaceae)植物分布范围广且属种多样,属种之间形态结构差异存在许多过渡类型,在属与种之间的鉴定上存在很多争议。目前,对于木兰科植物种子分类和鉴定研究中,通常以植物学形状特征作为鉴定方法。为了探索对木兰科植物种子进行鉴别的有效途径,采用酸性聚丙烯酰胺凝胶电泳对10种木兰科植物种子醇溶蛋白进行分析。结果发现,10种木兰科植物共分离出61条谱带,条带数大多在6~7,最多为9条,最少的6条。利用Quantityone软件对电泳谱带进行分析,发现不同种之间谱带都存在差异性,可以通过种子醇溶蛋白谱带进行木兰科植物物种鉴别。The Magnoliaceae plants are widely distributed and varied in genus and species.There are many controversies in the identification of genus and species because many transitional types were found among different genus and species.At present,botanical shape characteristics are usually used as identification methods for the classification and identification of magnoliaceae plants.In order to further identify the magnolia plants,the acid polyacrylamide gel electrophoresis was used to analyze the gliadin of magnolia plants.The results showed that 61 bands were separated from 10 species of magnolia,and most of the bands were between 6 and 7,with a maximum of 9,and the lowest 6.Using Quantityone software to analyze electrophoresis bands,it is found that there are differences between different bands,and species identification can be carried out through spectral bands.

关 键 词：木兰科(Magnoliaceae) 醇溶蛋白 聚丙烯酰胺凝胶电泳 

分 类 号：Q94[生物学—植物学]