数字PCR应用研究进展  

On the Current Research of Digital PCR Application

作　　者：王哲 雷舒文 段林洁 吕新瑞 古晓奎 WANG Zhe;LEI Shuwen;DUAN Linjie;LV Xinrui;GU Xiaokui(Guangdong Shunde Industry Design Institute(Guangdong Shunde Innovative Design Institute),Foshan Guangdong 528311,China)

机构地区：[1]广东顺德工业设计研究院/广东顺德创新设计研究院,广东佛山528311

出　　处：《顺德职业技术学院学报》2020年第1期1-4,11,共5页Journal of Shunde Polytechnic

摘　　要：数字PCR是一项基于PCR技术的全新核酸检测技术,其具有绝对定量、高灵敏度等优势,广泛用于体外诊断肿瘤稀有突变的绝对定量、HIV病毒载量,食品安全管控中对食源性病原菌定量,海关检验检疫对进口农产品进行转基因丰度的测定等。文章主要讨论微滴式数字PCR在食品安全和临床分子诊断领域的应用进展。As a novel nucleic acid test technique with absolute quantitation and high sensitivity,digital PCR is wildly applied in vitro diagnosis of the absolute quantitation of rare mutation in tumor,the number of HIV virus,the quantification of foodborne pathogens in food safety control,and the determination of transgenic abundance of imported agricultural products in Customs quarantine inspection.This article focuses on the current applications of microdrop digital PCR in the fields of food safety and clinical molecular diagnosis.

关 键 词：数字PCR 食品安全 绝对定量 病毒 肿瘤 

分 类 号：TS225.13[轻工技术与工程—粮食、油脂及植物蛋白工程]