Effects of mild intrauterine hypoperfusion in the second trimester on memory and learning function in rat offspring  

作　　者：Shao-Wei Yin Yuan Wang Yi-Lin Meng Cai-Xia Liu 

机构地区：[1]Department of Gynecology and Obstetrics,Shengjing Hospital of China Medical University,Shenyang,Liaoning Province,China [2]Key Laboratory of Maternal-Fetal Medicine of Liaoning Province,Shenyang,Liaoning Province,China [3]Department of Anesthesiology,Shengjing Hospital of China Medical University,Shenyang,Liaoning Province,China

出　　处：《Neural Regeneration Research》2020年第11期2082-2088,共7页中国神经再生研究（英文版）

基　　金：supported by the National Key Research&Department Program of China,No.2018YFC1002902(to CXL)。

摘　　要：Mild intrauterine hypoperfusion(MIUH)is a serious pathological event that affects the growth and development of fetuses and offspring.MIUH can lead to growth restriction,low birth weight,neurodevelopmental disorders,and other adverse clinical outcomes.To study the effects of MIUH on learning and memory function in offspring,a model of MIUH was established by placing a coil(length 2.5 mm,diameter 0.24 mm)on the uterine artery and ovarian uterine artery of Sprague-Dawley rats in the second trimester of pregnancy(day 17).Next,120 mg/kg lithium chloride(the MIUH+Li group)or normal saline(the MIUH group)was injected intraperitoneally into these rats.In addition,120 mg/kg lithium chloride(the Li group)or normal saline(the SHAM group)was injected intraperitoneally into pregnant rats without coil placement.The Morris water maze was used to detect changes in learning and memory ability in the offspring at 4 weeks after birth.In the MIUH group,the escape latency and journey length before reaching the platform were both increased,and the number of times that the platform was crossed and the activity time in the target quadrant within 90 seconds were both decreased compared with the SHAM group.Immunofluorescence double staining and western blot assays demonstrated that hippocampal nestin and Ki67(both cell-proliferation-related proteins)expression was significantly downregulated in the MIUH group compared with the SHAM group.Furthermore,western blot assays were conducted to investigate changes in related signaling pathway proteins in the brains of offspring rats,and revealed that glycogen synthase kinase 3β(GSK3β)expression was upregulated andβ-catenin expression was downregulated in the MIUH group compared with the SHAM group.In addition,compared with the MIUH group,the expression levels of p-GSK3βandβ-catenin were upregulated in the MIUH+Li group.These results suggest that MIUH may affect learning and memory function in rat offspring by regulating the GSK3βsignaling pathway.The experimental procedures were approved b

关 键 词：developmental NEUROBIOLOGY glycogen synthase kinase 3 INTRAUTERINE LEARNING lithium MEMORY OFFSPRING placenta diseases signal pathways β-catenin 

分 类 号：R714.51[医药卫生—妇产科学]