EDNRB基因真核表达载体的构建及其对MCF-7细胞增殖的影响  

Construction of EDNRB Gene Eukaryotic Expression Vector and Its Effect on Proliferation of MCF-7 Cells

作　　者：刘立琨 刘得水 李新 岳丽玲 林悦铭 张微 周丽 LIU Li-kun;LIU De-shui;LI Xin;YUE Li-ling;LIN Yue-ming;ZHANG Wei;ZHOU Li(Qiqihar Medical Institute of Pharmaceutical Sciences,Qiqihar,Heilongjiang Province,161006,China;Third Affiliated Hospital of Qiqihar Medical College Breast Surgery,Qiqihar,Heilongjiang Province,161000,China;Medical Technology School,Qiqihar Medical College,Qiqihar,Heilongjiang Province,161006,China)

机构地区：[1]齐齐哈尔医学院医药科学研究院,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院附属第三医院乳腺外科,黑龙江齐齐哈尔161000 [3]齐齐哈尔医学院医学技术学院,黑龙江齐齐哈尔161006

出　　处：《中外医疗》2020年第2期26-28,共3页China & Foreign Medical Treatment

基　　金：黑龙江省教育厅科学技术研究项目(2016-KYYWF-0863)。

摘　　要：目的构建pCI2-EDNRB真核表达载体,探讨EDNRB过表达对MCF-7细胞增殖的影响。方法设计EDNRB基因特异性引物,应用PCR法扩增其编码序列,构建pCI2-EDNRB真核表达载体并转染MCF-7细胞,RT-PCR和Western blot分别检测EDNRB的mRNA和蛋白表达;MTT法检测EDNRB过表达后MCF-7细胞增殖能力。结果pCI2-EDNRB真核表达载体成功构建,MCF-7细胞中EDNRB的mRNA和蛋白水平均较对照组明显升高,过表达EDNRB抑制了MCF-7细胞的增殖。结论该研究成功构建了pCI2-EDNRB真核表达载体,过表达EDNRB可抑制MCF-7细胞增殖,这为深入研究EDNRB在乳腺癌中的功能及分子机制打下良好基础。Objective To construct the eukaryotic expression vector pCI2-EDNRB and investigate the effect of EDNRB overexpression on the proliferation of MCF-7 cells.Methods EDNRB gene-specific primers were designed and amplified by PCR.The eukaryotic expression vector pCI2-EDNRB was constructed and transfected into MCF-7 cells.The mRNA and protein expression of EDNRB were detected by RT-PCR and Western blot,respectively.MTT assay The proliferation ability of MCF-7 cells after over-expression of EDNRB was examined.Results The eukaryotic expression vector of pCI2-EDNRB was successfully constructed.The mRNA and protein levels of EDNRB in MCF-7 cells were significantly higher than those in the control group.Overexpression of EDNRB inhibited the proliferation of MCF-7 cells.Conclusion The eukaryotic expression vector pCI2-EDNRB was successfully constructed in this study.Overexpression of EDNRB can inhibit the proliferation of MCF-7 cells,which lays a good foundation for further study of the function and molecular mechanism of EDNRB in breast cancer.

关 键 词：内皮素受体B 真核表达 MCF-7细胞 细胞增殖 

分 类 号：R736[医药卫生—肿瘤]