姜黄素对A549肺癌细胞增殖、侵袭和迁移的抑制作用及与Keap1/Nrf2信号通路的关系  

Inhibitory effect of curcumin on proliferation,invasion and migration of A549 lung cancer cells and its relationship with Keap1/Nrf2 signaling pathway

作　　者：金磊[1] 郭洁[2] 刘昊[1] 康云帆 徐长福[3] Jin Lei;Guo Jie;Liu Hao;Kang Yunfan;Xu Changfu(Department of Cardiothoracic Surgery,The First Affiliated Hospital of Xi’an Medical University,Xi’an 710077,China;Department of Emergency,The First Affiliated Hospital of Xi’an Medical University,Xi’an 710077,China;School of Basic Medical Sciences,Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安医学院第一附属医院心胸外科,西安710077 [2]西安医学院第一附属医院急诊科,西安710077 [3]西安交通大学医学院基础医学院,西安710061

出　　处：《中国组织化学与细胞化学杂志》2019年第6期501-507,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：陕西省教育厅课题(18JK0674)。

摘　　要：目的探究姜黄素对肺癌A549细胞增殖、侵袭和迁移的抑制作用及其机制。方法通过MTT法检测姜黄素对A549细胞增殖能力的影响;通过流式细胞术测定姜黄素对A549细胞凋亡的调节作用;通过Transwell试验,观察姜黄素对肺癌A549细胞的侵袭和迁移能力的影响;采用Western blot实验和RT-PCR实验,观察姜黄素对其Keap1/Nrf2信号通路的调节作用。结果增殖实验、凋亡实验和Transwell实验显示,姜黄素能够显著性抑制癌细胞的增殖、侵袭和迁移,并能够促进其凋亡。Western blot检测显示姜黄素能够显著抑制Keap1和Nrf2表达;RT-PCR实验结果显示姜黄素能够显著抑制Keap1mRNA和Nrf2 mRNA表达。结论姜黄素可能通过抑制Keap1/Nrf2信号通路蛋白的表达而抑制肺癌A549细胞的增殖、迁移和侵袭,并促进其凋亡。Objective To investigate the effect and mechanism of curcumin on proliferation,invasion and migration of lung cancer A549 cells.Methods MTT method was used to detect the effect of curcumin on the proliferation of A549 cells;Flow cytometry was used to measure the effect of curcumin on the apoptosis of A549 cells;Transwell test was used to evaluate the effect of curcumin on the invasion and migration of A549 cells.The influence of curcumin on expression of Keap1/Nrf2 signal pathway was quantitative analyzed by Western blot and RT-PCR.Results The results of the proliferation experiment,cell apoptosis assay and Transwell experiment showed that curcumin can significantly inhibit the proliferation,invasion and migration of A549 cells,and promote their apoptosis.Western blot showed that curcumin could significantly inhibit the expression of Keap1 and Nrf2 proteins;RT-PCR showed that curcumin could significantly inhibit the expression of Keap1 mRNA and Nrf2 mRNA.Conclusion Curcumin may inhibit the proliferation,migration and invasion of lung cancer A549 cells and promote their apoptosis by inhibiting the expression of Keap1/Nrf2 signaling pathway protein.

关 键 词：姜黄素 A549细胞 Keap1/Nrf2信号通路 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]