IL-8基因型多态性及其表达水平对鼻咽癌易感性的诊断价值  

Diagnostic Value of IL-8 Polymorphism and its Expression Level in the Susceptibility of Nasopharyngeal Carcinoma

作　　者：潘国刚[1] 杨碧秀[1] 王春芳[1] 许桂丹[1] 梁丽娜[1] 龙红兵[1] 邓益斌[1] PAN Guogang;YANG Bixiu;WANG Chunfang(Affiliated Hospital of Youjiang Nationality Medical College, Guangxi Baise 533000, China)

机构地区：[1]右江民族医学院附属医院,广西百色533000

出　　处：《河北医学》2020年第4期529-533,共5页Hebei Medicine

摘　　要：目的:分析鼻咽癌(NPC)患者白细胞介素-8(IL-8)基因型多态性及其表达水平,并探讨其在NPC易感性的诊断价值。方法:收集我院2017年1月至2019年9月住院的NPC患者共168例作为实验组,选取同期体检健康者232例为对照组(NC组),采用聚合酶链反应-限制片段长度多态(PCR-RFLP)方法对IL-8基因-251、+781、+1633及+2767位点进行多态性分析,采用酶联免疫吸附法(ELISA)检测NPC患者中血清IL-8的表达水平,利用logistic回归分析评价IL-8基因多态性位点与NPC易感性的关系,分析各基因型不同暴露因素在NPC易感性的相互作用,利用工作特异性曲线(ROC)评价IL-8水平对NPC的诊断价值。结果:与NC组比较,IL-8-251基因位点TT基因型(OR=1.98,95%CI:1.00~3.82)和TA基因型(OR=1.35,95%CI:1.01~2.61)患NPC的风险显著升高,AA基因型(OR=1.76,95%CI:1.02~3.08)患NPC的风险显著降低,NPC患者IL-8表达水平明显升高,差异有统计学意义(P<0.05);IL-8-251位点SNPs与吸烟、EBV感染因素在NPC的发生中存在负交互作用;IL-8水平对NPC易感性的曲线下面积(AUC)为0.852,敏感性为86%,特异性为78%。结论:IL-8基因-251位点基因多态性、血清IL-8表达升高与NPC易感性相关,且在吸烟、EBV感染暴露下发生交互作用,IL-8水平对NPC易感性具有诊断价值。Objective:To analyze the interleukin-8(IL-8)genotype polymorphism and its expression level in NPC patients,and to explore its diagnostic value in NPC susceptibility.Methods:A total of 168 NPC patients in our hospital from January 2017 to September 2019 were collected as the experimental group.232 healthy patients in the same period were selected as the control group(NC group).The polymorphism of IL-8 gene-251,+781,+1633 and+2767 were analyzed by PCR-RFLP,and the serum IL-8 in NPC patients was detected by ELISA The relationship between IL-8 gene polymorphism and NPC susceptibility was evaluated by logistic regression analysis.The interaction of different exposure factors in NPC susceptibility was analyzed.The diagnostic value of IL-8 level in NPC was evaluated by ROC.Results:Compared with the NC group,the risk of NPC in the IL-8-251 gene locus TT genotype(OR=1.98,95%CI:1.00~3.82)and AA genotype(OR=1.76,95%CI:1.02~3.08)was significantly increased,the risk of NPC in the TA genotype(OR=1.35,95%CI:1.01~2.61)was significantly reduced,and the expression level of IL-8 in NPC patients was significantly increased(P<0.05);There was a negative interaction between IL-8-251 SNPs and smoking and EBV infection factors in the occurrence of NPC.The area under the curve(AUC)of IL-8 susceptibility to NPC was 0.852,with 86%sensitivity and 78%specificity.Conclusion:The genotype polymorphism at the-251 locus of IL-8 gene and increased expression of serum IL-8 are related to NPC susceptibility,and interact with smoking and EBV infection exposure.IL-8 level is of diagnostic value for NPC susceptibility.

关 键 词：IL-8 基因多态性 鼻咽癌 

分 类 号：R73[医药卫生—肿瘤]