葫芦素D通过下调GATA6表达抑制SGC-7901胃癌细胞增殖迁移的作用机制研究  

The Mechanism of Cucurbitacin D inhibits Proliferation and Migration of SGC-7901 Gastric Cancer Cells by Down-regulating GATA6 Expression

作　　者：冯晓波[1] 李敏虹 尚精娟[1] 周颖[1] FENG Xiaobo;LI Minhong;SHANG Jingjuan(Shanghai Seventh People's Hospital, Shanghai 200136, China)

机构地区：[1]上海市第七人民医院胃肠疾病诊疗部,上海200136

出　　处：《河北医学》2020年第4期571-576,共6页Hebei Medicine

基　　金：上海市浦东新区卫生系统学科带头人培养计划,(编号:PWRd2016-12)。

摘　　要：目的:探讨葫芦素D通过下调GATA转录因子6(GATA6)表达抑制SGC-7901胃癌细胞增殖、迁移的作用机制。方法:体外培养SGC-7901细胞,用MTT法检测葫芦素D对SGC-7901细胞抑制率并确定葫芦素D的干预剂量,将细胞分为对照组、顺铂组、葫芦素D低、中、高剂量组。对照组不进行处理,顺铂组给予终浓度为10.0μmoL/L的顺铂,葫芦素D低、中、高剂量组分别给予终浓度为2.0、4.0、8.0μmoL/L的葫芦素D,继续培养48h后进行检测SGC-7901细胞迁移能力、SGC-7901细胞中miRNA-143和GATA6 mRNA表达水平及细胞凋亡和细胞周期情况测定。结果:随着葫芦素D剂量增加,SGC-7901细胞抑制率增加(P<0.05)。在葫芦素剂量为8.0μmoL/L时,抑制率为53.07%,故选8.0μmoL/L作为最高干预剂量,并设2.0μmoL/L和4.0μmoL/L两个剂量。与对照组比较,顺铂组SGC-7901细胞迁移能力、GATA6 mRNA相对表达量和G0/G1期细胞比例降低,miRNA-143 mRNA的相对表达量、细胞凋亡率和S期细胞比例增加(P<0.05);给予葫芦素D处理后,SGC-7901细胞迁移能力、GATA6 mRNA相对表达量和G0/G1期细胞比例降低,miRNA-143 mRNA的相对表达量、细胞凋亡率和S期细胞比例增加,且呈剂量依赖性(P<0.05),但效果不如顺铂组。结论:葫芦素D能抑制SGC-7901胃癌细胞增殖和迁移,具有一定的抗癌活性,其机制可能与葫芦素D通过下调GATA6表达有关。Objective:To investigate the mechanism of cucurbitacin D inhibits proliferation and migration of SGC-7901 gastric cancer cells by down-regulating GATA6 expression.Methods:SGC-7901 cells were cultured in vitro.The inhibition rate of cucurbitacin D on SGC-7901 cells was detected by MTT method and the intervention dose of cucurbitacin D was determined.The cells were divided into control group,cisplatin group,cucurbitacin D low,medium and high dose groups.The control group was not treated.The cisplatin group was given cisplatin with a final concentration of 10.0μmoL/L.The low,medium and high dose groups were given cucurbitacin D with the final concentration of 2.0,4.0,8.0μmoL/L,respectively.The migration ability of SGC-7901 cells,the expression level of mi-R143 and GATA6 in SGC-7901 cells and the apoptosis and cell cycle were detected after 48 hours of culture.Results:With the increase of cucurbitacin D dose,the inhibition rate of SGC-7901 cells was significantly increased(P<0.05).When the dose of cucurbitacin was 8.0μmoL/L,the inhibition rate was 53.07%.Therefore,8.0μmoL/L was chosen as the highest intervention dose,and 2.0μmoL/L and 4.0μmoL/L were set as two doses.Compared with the control group,the migration ability of SGC-7901 cells,the relative expression of GATA6 mRNA,and the proportion of G0/G1 cells in the cisplatin group were significantly decreased,and the relative expression of miRNA-143 mRNA,the apoptosis rate,and the proportion of S-phase cells significantly increased(P<0.05).After treatment with cucurbitin D,the migration ability of SGC-7901 cells,the relative expression of GATA6 mRNA,and the ratio of G0/G1 cells were significantly decreased,and the relative expression of miRNA-143 mRNA,the rate of apoptosis,and the proportion of S cells significantly increased with dose-dependent(P<0.05),but the effect was not as good as the cisplatin group(P<0.05).Conclusion:Cucurbitacin D can inhibit the proliferation and migration of SGC-7901 gastric cancer cells,and has certain anti-cancer activity.The mechan

关 键 词：葫芦素D GATA6 SGC-7901胃癌细胞 细胞增殖 细胞迁移 

分 类 号：R73[医药卫生—肿瘤]