镉铜单一及复合胁迫对双齿围沙蚕(Perineresis aibuhitensis)消化酶活性和抗氧化指标的影响  

EFFECTS OF SINGLE Cu,Cd,AND Cu-Cd COMBINED STRESS ON DIGESTIVE ENZYME ACTIVITIES AND ANTIOXIDANT INDEXES OF PERINEREIS AIBUHITENSIS

作　　者：陈强[1,2] 黎中宝 高瞻[1,2] CHEN Qiang;LI Zhong-Bao;GAO Zhan(Fisheries College,Jimei University,Xiamen 361021,China;Fujian Provincial Key Laboratory ofMarine Fishery Resources and Eco-environment,Xiamen 361021,China)

机构地区：[1]集美大学水产学院,厦门361021 [2]福建省海洋渔业资源与生态环境重点实验室,厦门361021

出　　处：《海洋与湖沼》2020年第2期395-404,共10页Oceanologia Et Limnologia Sinica

基　　金：福建省中青年教师教育科研项目,JT180275号;集美大学学科建设基金项目,ZC2018021号,集大科【2019】21号;福建省海洋渔业资源与生态环境重点实验室开放基金资助项目,ZK2014005号;福建省自然科学基金项目,2017J01638号。

摘　　要：采用实验生态学方法,研究了Cd2+-Cu2+单一及复合胁迫3d、6d、9d对双齿围沙蚕(Perinereis aibuhitensis)消化酶活性和抗氧化指标的影响。结果表明,沙蚕的各项不同指标对Cd2+、Cu2+胁迫的响应表现出不同的变化趋势,复合胁迫中Cd2+-Cu2+对沙蚕各指标的影响存在交互效应。沙蚕3种消化酶活性大小为淀粉酶>蛋白酶>脂肪酶。Cd2+、Cu2+单一胁迫能激发沙蚕淀粉酶活性,激发作用强于复合胁迫。Cd2+、Cu2+单一胁迫实验前期低浓度组蛋白酶被抑制,高浓度组被诱导,复合胁迫实验前期Cd2+-Cu2+对蛋白酶活性的影响存在协同作用。Cd2+、Cu2+胁迫对沙蚕脂肪酶活性整体表现出抑制作用。Cd2+、Cu2+胁迫诱导沙蚕超氧化物歧化酶(SOD),整体上激活过氧化氢酶(CAT)活性。Cd2+单一胁迫实验组过氧化物酶(POD)均被显著诱导,具有"剂量-效应"关系,Cu2+单一胁迫实验后期POD被显著诱导,复合胁迫实验中,低浓度Cu2+组POD被显著诱导,高浓度Cu2+组POD被显著抑制。实验组沙蚕谷胱甘肽过氧化物酶(GXH-Px)活性和丙二醛(MDA)含量均高于对照组,复合胁迫实验前期,GXH-Px活性与Cu2+浓度呈正相关关系,与Cd2+浓度呈负相关关系。显著的效应-剂量间相关关系存在于特定时间(3d、6d)的MDA含量,沙蚕MDA含量用作监测海洋重金属污染的生物标志物的可能性较大。An experimental ecology method was conducted to investigate digestive enzyme activities and antioxidant indexes of Perinereis aibuhitensis exposed to different concentrations of heavy metal ions Cd2+,Cu2+,and their combination(Cd2+-Cu2+)from 3 d to 9 d.Results show that the factors of Perinereis aibuhitensis show different variation patterns under different concentrations of metal ions,and all factors were affected by the interaction effect of Cd2+and Cu2+in the combined stress experiments.The activity of amylase in P.aibuhitensis was the highest among the three digestive enzymes,and the activity of protease was higher than that of lipase.Single Cd2+and Cu2+could stimulate amylase to cope with the stress environment,and stimulation of single Cd2+and Cu2+stress on amylase was stronger than that of Cu-Cd combined stress.Compared with the control group,when P,aibuhitensis were exposed to low concentrations of single Cd2+and Cu2+,their protease activities decreased in the early experimental period;when exposed to high concentrations of single Cd2+and Cu2+,their protease activities increased in the early experimental period.There was a synergy interaction of Cd2+and Cu2+on protease activities in the early period of combined stress experiment.Treatment with Cd2+and Cu2+had an inhibitory effect on lipase activities.Cd2+and Cu2+stress showed inductive effect on the activities of the SOD and CAT.POD activities in the single Cd2+stress groups were significantly induced and positively correlated with the Cd2+stress intensity.In the later period of the experiment,the POD activities in the single Cu2+stress groups significantly increased.In the combined stress experimental process,the POD activities in the low concentration of Cu2+groups were significantly induced,and those in the high concentration of Cu2+groups were significantly inhibited.GXH-Px activities and MDA contents in all experimental groups were significantly higher than those of the control group.In the combined stress experimental process,GXH-Px activities had o

关 键 词：重金属 双齿围沙蚕 胁迫 消化酶 抗氧化指标 

分 类 号：X171.5[环境科学与工程—环境科学] X503.22