肺癌患者氧化损伤与趋化因子IP-10、Rantes的相关性研究  

Correlationship between oxidative damage and IP-10,Rantes in lung cancer patients

作　　者：陈吉杰 崔华英 魏春华 赵静渝 Chen Jijie;Cui Huaying;Wei Chunhua;Zhao Jingyu(Department of Geriatrics,People′s Hospital of Dongying District,Dongying 257085,China;Department of Respiratory Medicine,Weien Hospital of Weifang City,Weifang 261041,China)

机构地区：[1]东营市东营区人民医院老年病科,257085 [2]潍坊市潍坊卫恩医院呼吸科,261041

出　　处：《国际呼吸杂志》2020年第7期516-520,共5页International Journal of Respiration

摘　　要：目的研究肺癌患者氧化应激水平和血清趋化因子γ-干扰素诱导蛋白10(IP-10)、T细胞表达分泌的活化调节因子(Rantes)含量的改变,探讨氧化损伤和趋化因子在肺癌发病机制中的作用及相互关系。方法收集2018年2月至2019年2月东营市东营区人民医院住院的52例肺癌患者为肺癌组,34例慢性支气管炎患者为配比组,20名健康者为健康对照组,采用黄嘌呤氧化酶法测定血清超氧化物歧化酶(SOD)活性,硫代巴比妥酸比色法测定丙二醛(MDA)含量,酶联免疫吸附法检测血清趋化因子IP-10及Rantes表达水平,并进行相关性分析。结果肺癌组SOD活性低于配比组和健康对照组(F=29.681,P<0.05)。肺癌组MDA含量高于配比组和健康对照组(F=61.151,P<0.05)。肺癌Ⅰ~Ⅳ期患者血清SOD活性依次降低,差异有统计学意义(F=11.734,P<0.05)。肺癌组血清IP-10及Rantes水平高于配比组和健康对照组(F=22.115、13.181,P<0.05)。SOD活性与IP-10及Rantes水平呈负相关性(r=-0.309、-0.376,P=0.017、0.034),MDA含量与IP-10水平呈正相关性(r=0.357,P=0.021)。结论氧化应激和趋化因子相互作用,可能在肺癌的发生、发展机制中发挥了重要作用。Objective To investigate the possible associations of oxidative damage and serum chemokines,IFN-γ-inducible protein-10(IP-10),Regulated on activation normal T-cell-expressed and secreted(Rantes)in lung cancer patients.Methods Blood samples were collected from 52 lung cancer patients as lung cancer group,34 chronic bronchitis patients as the ratio of group and 20 healthy controls as healthy control group Who were admitted to the department of geriatrics of Dongying district people′s hospital from February 2018 to February 2019.The activities of serum superoxide dismutase(SOD)were measured by xanthine oxidase method,the levels of malondialdehyde(MDA)were detected by thiobarbituric acid colorimetric method(TBA)and the serum levels of IFN-γ-inducible protein-10(IP-10),regulated on activation normal T-cell-expressed and secreted(Rantes)were measured by enzyme-linked immunosorbent assay(ELISA).Linear correlation analysis was used to analyze the correlationship.Results The activities of serum SOD were significantly lower in lung cancer group compared to the ratio of group and healthy control group(F=29.681,P<0.05).while the levels of MDA were significantly higher in lung cancer group compared to the ratio of group and healthy control group(F=61.151,P<0.05).In addition,the activities of SOD were correlated to clinical stages in patients with lung cancerⅠ~Ⅳperiod,the difference was statistically significant(F=11.734,P<0.05).The levels of IP-10 and Rantes were higher in lung cancer group than the ratio of group and healthy control group(F=22.115,13.181,P<0.05).The levels of IP-10 and Rantes were negatively correlated with the activities of SOD(r=-0.309,-0.376,P=0.017,0.034),while positively correlated with the levels of MDA(r=0.357,P=0.021).Conclusions Strong associations were found between chemokines and oxidative stress which participated in the pathogenesis of lung cancer.

关 键 词：肺肿瘤 超氧化物歧化酶 丙二醛 γ-干扰素诱导蛋白10 T细胞表达分泌的活化调节因子 

分 类 号：R734[医药卫生—肿瘤]