miR-424对肝癌细胞增殖、细胞周期和侵袭活性的影响  

Effect of MicroRNA-424 on Proliferation,Cell Cycle and Invasive Activity in Liver Cancer Cells

作　　者：吕薇 龙波 魏秀丽 LYU Wei;LONG Bo;WEI Xiu-li(Department of Laboratory,the Second Naval Hospital of Southern Theater,Sanya,Hainan 572000,China)

机构地区：[1]南部战区海军第二医院检验科,海南三亚572000

出　　处：《解放军医药杂志》2020年第4期33-35,共3页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

摘　　要：目的观察微小RNA-424(miR-424)对肝癌细胞增殖、细胞周期和侵袭活性的影响。方法肝癌HepG2细胞转染miR-424表达质粒,同时设立空质粒载体转染阴性对照组和无转染的空白对照组。荧光定量PCR检测miR-424表达水平,克隆形成实验检测细胞增殖,流式细胞仪检测细胞周期,Transwell实验检测侵袭能力。结果miR-424表达组2-ΔΔCt值、G1期细胞比例显著高于空白对照组和阴性对照组,克隆形成率和穿过Transwell小室细胞数低于空白对照组和阴性对照组(P<0.05,P<0.01)。miR-424表达组S期和G2期细胞比例低于空白对照组和阴性对照组(P<0.05)。结论过表达miR-424可以抑制肝癌HepG2细胞增殖和侵袭能力。Objective To observe the effect of microRNA-424(miR-424)on proliferation,cell cycle and invasive activity in liver cancer cells.Methods HepG2 cells were transfected with miR-424 expression plasmid.The empty plasmid transfection control group(negative control group)and non-transfection control group(blank control group)were set up.The expression of miR-424 was detected by real-time PCR assay.Cell proliferation was detected by clonogenic assay.The cell cycle was detected by flow cytometry assay,and the invasive activity was detected by Transwell assay.Results The 2-ΔΔCt value and G1 phase cell proportion and cell number were significantly higher,while the clone formation rate and the number of cells passing through the Transwell chamber were lower in miR-424 expression group,as compared with those in blank control and negative control groups(P<0.05,P<0.01).The proportion of cells at S phase and G2 phase in miR-424 expression group were lower than that in blank control group and negative control group(P<0.05).Conclusion Overexpression of miR-424 can inhibit proliferation and invasion of HepG2 liver cancer cells.

关 键 词：微小RNA-424 肝肿瘤 细胞增殖 细胞周期 肿瘤侵润 

分 类 号：R735.7[医药卫生—肿瘤]