响应面法优化皮状丝孢酵母产油脂发酵培养基  

Optimization of fermentation medium for oil production by Trichosporon cutaneum using response surface methodology

作　　者：许鹏飞 程志敏[2] 胡裕升 朱玉博 杨锟 任立伟 龚大春[1,2] XU Pengfei;CHENG Zhimin;HU Yusheng;ZHU Yubo;YANG Kun;REN Liwei;GONG Dachun(Key Laboratory of Functional Yeast,China National Light Industry,China Three Gorges University,Yichang 443002,China;Hubei Engineering Research Center for Biological Jiaosu,China Three Gorges University,Yichang 443002,China)

机构地区：[1]三峡大学中国轻工业功能酵母重点实验室,湖北宜昌443002 [2]三峡大学湖北省生物酵素工程技术研究中心,湖北宜昌443002

出　　处：《中国酿造》2020年第3期94-98,共5页China Brewing

基　　金：国家自然科学基金项目(21776162);三峡大学学位论文培优基金项目资助(2019SSPY151)。

摘　　要：以皮状丝孢酵母(Trichosporon cutaneum)为出发菌株,对其产油脂的发酵培养基进行研究。以油脂产量为评价指标,通过单因素试验研究发酵培养基中的碳源、氮源、外源因子对油脂产量的影响,然后利用响应面试验对培养基进行优化。结果表明,最佳培养基配方为葡萄糖97.6 g/L、玉米浆干粉4.4 g/L、乙酸钠0.09 g/L。在该优化条件下,皮状丝孢酵母的油脂产量达到了14.4 g/L。Using Trichosporon cutaneum as starting strain,the fermentation medium for oil production was studied.Using oil yield as evaluation indicator,the effects of carbon source,nitrogen source and exogenous factors of the fermentation medium on the oil yield were studied by single factor experiments.Then the medium was optimized by response surface experiments.The results showed that the optimal medium formula was obtained as follows:glucose 97.6 g/L,corn syrup dry powder 4.4 g/L,and sodium acetate 0.09 g/L.Under the optimal conditions,the oil yield of T.cutaneum reached 14.4 g/L.

关 键 词：皮状丝孢酵母 培养基 响应面法 微生物油脂 

分 类 号：TQ642[化学工程—精细化工] Q939.9[生物学—微生物学]