miR-155-5p和ELK3在胆囊癌组织中的表达及其临床意义  

The expressions and clinical significances of miR-155-5p and ELK3 in gallbladder carcinoma tissues

作　　者：王思贺 Wang Sihe(Department of General Surgery,Zaozhuang Mining Group Zaozhuang Hospital,Shandong Zaozhuang 277100,China)

机构地区：[1]枣庄矿业集团枣庄医院普外科,山东枣庄277100

出　　处：《现代肿瘤医学》2020年第10期1707-1711,共5页Journal of Modern Oncology

摘　　要：目的:分析miR-155-5p和ELK3在胆囊癌组织中的表达水平,并探讨其表达与胆囊癌临床病理特征及预后的关系。方法:收集2014年1月至2015年5月在本院进行手术治疗的45例胆囊癌患者的肿瘤组织和癌旁非肿瘤组织,采用RT-PCR检测组织中miR-155-5p和ELK3 mRNA表达水平,采用免疫组织化学染色法检测组织中ELK3蛋白表达情况,分析肿瘤组织中miR-155-5p和ELK3表达水平的关系,分析miR-155-5p和ELK3表达水平与胆囊癌患者肿瘤大小、转移、浸润等病理特征及3年总生存率的关系。结果:肿瘤组织中miR-155-5p、ELK3 mRNA表达水平及ELK3蛋白阳性率均高于癌旁非肿瘤组织(P<0.05);肿瘤组织中miR-155-5p和ELK3表达水平呈正相关(P=0.002);miR-155-5p表达水平与肿瘤大小、组织分化程度、淋巴结转移、远处转移、血管浸润、胆管侵犯、肝脏侵犯有关(P<0.05),ELK3阳性率与FIGO分期、淋巴结转移、血管浸润、胆管侵犯和肝脏侵犯有关(P<0.05);miR-155-5p高表达组患者3年总生存率低于miR-155-5p低表达组患者(P<0.05),ELK3阳性表达患者3年生存率低于ELK3阴性表达患者(P<0.05)。结论:胆囊癌组织中miR-155-5p和ELK3表达上调,与胆囊癌转移、侵袭等恶性生物学行为及不良预后密切相关,可作为胆囊癌临床预后预测的检测指标。Objective:To analyze the expressions of miR-155-5p and ELK3 in gallbladder carcinoma and to explore their relationship with clinicopathological features and prognosis of gallbladder carcinoma.Methods:From January 2014 to May 2015,the tumor tissues and adjacent non-tumor tissues of 45 patients with gallbladder cancer underwent surgical treatment in our hospital were collected.The expression levels of miR-155-5p and ELK3 mRNAs in tissues were detected by RT-PCR.Immunohistochemical staining was used to detect the expression of ELK3 protein in tissues.The relationship between the expressions of miR-155-5p and ELK3 in tumor tissues was analyzed,and the relationships between the expressions of miR-155-5p and ELK3 with the pathological features of tumor size,metastasis and invasion in patients with gallbladder carcinoma and the 3-year overall survival rate were analysed.Results:The expression levels of miR-155-5p,ELK3 mRNA and the positive rate of ELK3 protein in tumor tissues were higher than those in non-tumor tissues(P<0.05).The expression levels of miR-155-5p and ELK3 in tumor tissues were positively correlated(P=0.002).miR-155-5p was associated with tumor size,histological differentiation,lymph node metastasis,distant metastasis,vascular invasion,bile duct invasion and liver invasion(P<0.05).ELK3 was associated with FIGO staging,lymph node metastasis,vascular invasion,bile duct invasion and liver invasion(P<0.05).The 3-year overall survival rate of miR-155-5p high expression group was lower than that of miR-155-5p low expression group(P<0.05),and the 3-year overall survival rate of ELK3 positive expression patients was lower than that of ELK3 negative expression patients(P<0.05).Conclusion:The expressions of miR-155-5p and ELK3 are up-regulated in gallbladder carcinoma tissues,which are closely related to malignant biological behaviors such as metastasis,invasion of gallbladder cancer and poor prognosis,and can be used as a prognostic indicators for clinical prognosis of gallbladder cancer.

关 键 词：胆囊癌 miR-155-5p ELK3 临床病理特征 预后 

分 类 号：R735.8[医药卫生—肿瘤]