髓系来源的抑制性细胞在小鼠H22原位种植性肝癌中的表达及其意义  

The expression and significance of myeloid-derived suppressor cells in murine H22 orthotopic transplantable hepatoma

作　　者：李宝华 孙晋 张晨[2] 秦博文 杨军[3] 孔光耀 李宗芳[2,4] Li Baohua;Sun Jin;Zhang Chen;Qin Bowen;Yang Jun;Kong Guangyao;Li Zongfang(Core Research Laboratory,Cadre Ward,the Second Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710004,China;National&Local Joint Engineering Research Center of Biodiagnosis and Biotherapy,Cadre Ward,the Second Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710004,China;Department of Pathology,Cadre Ward,the Second Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710004,China;Department of Surgery,Cadre Ward,the Second Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710004,China)

机构地区：[1]西安交通大学第二附属医院科研中心实验室,陕西西安710004 [2]西安交通大学第二附属医院生物诊断与生物治疗国家地方联合工程研究中心,陕西西安710004 [3]西安交通大学第二附属医院病理科,陕西西安710004 [4]西安交通大学第二附属医院普通外科干部病房,陕西西安710004

出　　处：《现代肿瘤医学》2020年第9期1419-1425,共7页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:81001309);陕西省重点研发计划一般项目-社会发展领域(编号:2018SF-197)。

摘　　要：目的:比较小鼠H22原位种植性肝癌模型中髓系来源的抑制性细胞(MDSCs)和调节性T细胞(Tregs)的表达,确定主要的免疫抑制性细胞。并探讨主要免疫抑制性细胞在小鼠H22原位种植性肝癌模型中的意义。方法:建立小鼠H22原位种植性肝癌模型,用流式细胞术的方法比较外周血、脾脏、肿瘤组织中MDSCs、Tregs的比例。分析MDSCs与CD4^+T细胞、CD8^+T细胞、NK细胞、NKT细胞的相关性。通过脾脏切除的方法下调MDSCs的表达,观察对肿瘤存活的影响。结果:MDSCs的比例无论在外周血、脾脏还是肿瘤组织中都显著高于Tregs。MDSCs的比例与CD4^+T细胞、CD8^+T细胞、NK细胞、NKT细胞呈负相关。下调MDSCs的比例可以显著延长小鼠存活期。结论:小鼠H22原位种植性肝癌中主要的免疫抑制性细胞是MDSCs。MDSCs的高表达预示着不良的小鼠存活期。Objective:To compare the percentages of myeloid-derived suppressor cells(MDSCs)and regulatory T cells(Tregs)and to verify which cell types are the main immune suppressive cells in murine H22 orthotopic transplantable hepatoma.Then,to discuss their significance in hepatoma mice.Methods:Murine H22 orthotopic transplantable hepatoma was established.The percentages of MDSCs and Tregs in peripheral blood,spleen and tumor tissue were compared using flow cytometry.The correlations between MDSCs and CD4^+T cells,CD8^+T cells,NK cells and NKT cells were analyzed.The effect of down-regulation of MDSCs using splenectomy on tumor survival was also detected.Results:The percentages of MDSCs were much higher than that of Tregs in the peripheral blood,spleen and tumor tissue.The percentages of MDSCs were negatively correlated with that of CD4^+T cells,CD8^+T cells,NK cells and NKT cells.The down-regulation of MDSCs significantly prolonged tumor survival.Conclusion:The main immune suppressive cell type was MDSCs in murine H22 orthotopic transplantable hepatoma.The higher expression of MDSCs predicted poorer tumor survival.

关 键 词：髓系来源的抑制性细胞 调节性T细胞 H22 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]