RNA干扰沉默Bmi-1基因对胃癌细胞BGC-823生长及顺铂敏感性作用的研究  

Effects of RNA interference silencing Bmi-1 gene on proliferation,invasion and cisplatin sensitivity of human gastric cancer BGC-823 cells

作　　者：王淼舟[1] 张弢 邓赢芳 柴海萍 柳仪 Wang Miaozhou;Zhang Tao;Deng Yingfang;Chai Haiping;Liu Yi(Affiliated Hospital of Qinghai University,Qinghai Xining 810001,China;the Second People's Hospital of Xining City,Qinghai Xining 810000,China)

机构地区：[1]青海大学附属医院,青海西宁810001 [2]西宁市第二人民医院,青海西宁810000

出　　处：《现代肿瘤医学》2020年第9期1433-1436,共4页Journal of Modern Oncology

基　　金：青海省科技计划项目(编号:2018-ZJ-T02);青海大学中青年科研基金项目(编号:2016-QYY-11)。

摘　　要：目的:探讨Bmi-1基因对胃癌细胞BGC-823增殖、侵袭及顺铂耐药的作用及可能机制。方法:采用LipofectamineTM2000向胃癌细胞BGC-823随机转染成功构建靶向沉默Bmi-1表达的siRNA,分别利用Western Blot、CCK-8、Transwell小室等方法对转染Bmi-1 siRNA的BGC-823细胞的增殖、侵袭能力及顺铂耐药进行检测。结果:转染Bmi-1 siRNA后,BGC-823细胞的增殖、侵袭能力显著下降(P<0.05),而且顺铂敏感性显著提高。结论:Bmi-1基因与胃癌BGC-823细胞系的增殖、侵袭及顺铂耐药相关,可能是胃癌治疗的靶点。Objective:To explore the effects of Bmi-1 on proliferation,invasion and cisplatin resistance of human gastric cells BGC-823,and discuss the possible mechanisms.Methods:siRNA targeting silencing Bmi-1 expression was transfected into gastric cancer cell line BGC-823 by LipofectamineTM2000.The change of proliferation,invasion and cisplatin sensitivity of gastric cancer cells was detected by Western Blot,CCK-8 and Transwell.Results:After being transfected by Bmi-1 siRNA,the proliferation and invasion ability of BGC-823 cells decreased significantly(P<0.05),and the sensitivity to cisplatin increased significantly.Conclusion:Bmi-1 plays roles on promoting cell proliferation,invasion,and cisplatin resistance of gastric cancer cells BGC-823.

关 键 词：BMI-1 胃癌细胞 SIRNA 顺铂 

分 类 号：R735.2[医药卫生—肿瘤]