结肠癌组织中的KLF8表达及下调KLF8对肠癌细胞的影响  

Expression of transcription factor KLF8 in colorectal cancer tissue and its effect of downregulation KLF8 on colorectal cancer cell

作　　者：严清青 王龙 黄耀星[1] 余丹纯[1] 刘静[1] YAN Qingqing;WANG Long;HUANG Yaoxing;YU Danchun;LIU Jing(Department of Gastroenterology,Guangzhou First People's Hospital,Guangzhou 510180,China;Department of Interventional Radiology,Guangzhou First People's Hospital,Guangzhou 510180,China)

机构地区：[1]广州市第一人民医院消化内科,广州510180 [2]广州市第一人民医院介入放射科,广州510180

出　　处：《广州医药》2020年第2期21-26,共6页Guangzhou Medical Journal

摘　　要：目的研究结肠癌组织中转录因子KLF8的表达及下调KLF8的表达对结肠癌细胞的影响。方法收集结肠癌组织和癌旁正常组织,检测KLF8的蛋白含量;培养结肠癌Lovo细胞株,转染KLF8 siRNA后检测细胞侵袭、迁移以及上皮-间质转化(EMT)。结果结肠癌组织中KLF8的蛋白含量高于癌旁正常组织;转染KLF8 siRNA的结肠癌细胞组迁移距离低于阴性对照组,且侵袭至transwell微孔膜外侧面的细胞数少于阴性对照组;转染KLF8 siRNA的结肠癌细胞组内E-cadherin的表达升高,Vimentin、N-cadherin的蛋白含量低于阴性对照组。结论结肠癌组织中KLF8的表达量升高,下调结肠癌细胞中KLF8的表达可抑制结肠癌细胞侵袭、迁移及上皮-间质转化过程。Objective To study the expression of transcription factor KLF8 in colorectal cancer tissue and its effect of downregulation KLF8 on colorectal cancer cell.Methods Collecting cancer tissues and adjacent normal color tissue and detecting the protein level of KLF8.Culturing the colorectal cancer Lovo cell lines and detecting cell invasion,cell migration and epithelial-mesenchymal transition after transfecting of KLF8 siRNA.Results KLF8 was highly expressed in colorectal cancer tissues compared with adjacent normal colon tissue.After transfection of KLF8 siRNA,the migration distance of colorectal cancer cell and the cell population transferred to the lateral surface of transwell microporous membrane were lower than those of negative control siRNA.E-cadherin of KLF8 siRNA group were higher than those of negative control siRNA group.Vimentin and N-cadherin were lower than those of negative control siRNA group.Conclusion The expression of KLF8 in colorectal cancer tissue is elevated;downregulation of KLF8 expression in colorectal cancer cell lines may inhibit cell invasion,cell migration and epithelial-mesenchymal transition processes.

关 键 词：结肠癌 KLF8 侵袭 迁移 上皮-间质转化 

分 类 号：R735.35[医药卫生—肿瘤]