缓释生长因子羧甲基壳聚糖支架抑菌功能的研究  

Study on antibacterial function of carboxymethyl chitosan scaffold for slow release growth factor

作　　者：李蕾[1,2] 林放 施琳颖 周谋 许育兵 李荣娟 赵春林 单桂秋 LI Lei;LIN Fang;SHI Linying;ZHOU Mou;XU Yubing;LI Rongjuan;ZHAO Chunlin;SHAN Guiqiu(Southern Medical University,Guangzhou,Guangdong 510515,China;Department of Blood Transfusion,General Hospital of Southern Theater Command of PLA,Guangzhou,Guangdong 510010,China;Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510405,China;Clinical Laboratory,Lixian People′s Hospital,Changde,Hunan 415500,China)

机构地区：[1]南方医科大学,广州510515 [2]中国人民解放军南部战区总医院输血科,广州510010 [3]广州中医药大学,广州510405 [4]湖南省常德市澧县人民医院检验科,415500

出　　处：《重庆医学》2020年第8期1212-1217,共6页Chongqing medicine

摘　　要：目的制备缓释生长因子的羧甲基壳聚糖支架(PRP/CMCS)并检测其抑菌功能。方法利用冻干技术构建一种富含多种生长因子的PRP/CMCS,观察支架的结构,ELISA法检测生长因子负载率和缓释效果,并通过抑菌圈试验和糖尿病合并细菌小鼠创面模型验证其抑菌功能。结果PRP/CMCS的孔径在20~125μm的范围内,孔隙率大于80%。血管内皮生长因子(VEGF)及血小板衍生生长因子(PDGF)第1天的负载率分别为35.7%和4.7%,体外缓释7 d累计释放量分别占总负载率的48.7%和14.6%。抑菌圈试验证明PRP/CMCS对大肠埃希菌和金黄色葡萄球菌具有明显抑制作用;动物实验也证实了其抑菌效果及具有促进难愈创面愈合的功能。结论PRP/CMCS具有稳定的抑菌功能,可作为一种高效可靠的抗感染促愈合材料。Objective To get a carboxymethyl chitosan scaffold(PRP/CMCS)for slow-release growth factors and test its antibacterial function.Methods A carboxymethyl chitosan scaffold rich in various growth factors was constructed by freeze-drying technology.The structure of the scaffold was detected,the loading rate of growth factors and slow-release effect were detected by ELISA.Inflammation tests of diabetic rats combined with bacterial wound and antibacterial circle test were detected for antibacterial function.Results The pore diameter of the PRP/CMCS were in the range of 20-125μm,and the porosity was more than 80%.The loading rates of VEGF and PDGF growth factors on the first day were 35.7%and 4.7%,respectively,and the cumulative release amount of 7 d sustained-release in vitro accounted for 48.7%and 14.6%of the total loading rate,respectively.The antibacterial circle test proved that the scaffold had obvious inhibitory effect on Escherichia coli and Staphylococcus aureus.Animal experiments also confirmed its antibacterial effect of promoting healing wounds.Conclusion The scaffold has stable bacteriostatic function and can be used as an efficient and reliable anti-infection and pro-healing material.

关 键 词：生长因子 羧甲基壳聚糖 支架 缓释 抑菌 

分 类 号：R453.9[医药卫生—治疗学]