人肌源性干细胞的生物特性及其治疗假肥大肌营养不良症模型鼠的初步研究  

Biological properties of human muscle-derived stem cells and its treatment for Duchenne muscular dystrophy in mdx mice:A preliminary study

作　　者：张惠丽 成秋生 陈希 李泽 ZHANG Huili;CHENG Qiusheng;CHEN Xi;LI Ze(Department of Neurology,Guangzhou First People′s Hospital,School of Medicine,South China University of Technology,Guang?zhou 510180,China)

机构地区：[1]广州市第一人民医院,华南理工大学附属第二医院神经科,广州510180

出　　处：《实用医学杂志》2020年第7期880-884,共5页The Journal of Practical Medicine

基　　金：广东省自然科学基金(编号:2017A030310619)。

摘　　要：目的观察人肌源性干细胞(MDSCs)的生物特性及其治疗假肥大肌营养不良症模型鼠(mdx)的再生潜能。方法预贴壁法分离细胞,采用免疫荧光,Real-time PCR和Western Blotting法检测相关成肌因子;并观察其移植治疗mdx后的dystrophin蛋白的变化。结果与D1-hMDCs相比,D6-hMDCs和D8-hMDCs的CD56、CD45和CD184均呈低表达;desmin和MHC呈显著高表达;移植到mdx鼠后,均能增强dystrophin蛋白表达,但D6-hMDCs与D8-hMDCs间没有差异。结论 D6-hMDCs和D8-hMDCs具有相似的生物学特性,属MDSCs,均比D1-hMDCs具有更强的成肌分化能力和再生潜能。Objective To identify the biological characteristics and the regenerative potential of human muscle-derived stem cells(hMDSCs)in the treatment of mdx mice. Methods The expression of myogenic markers were detected via immunofluorescence(IF),Real-time PCR,and Western Blotting. After cell transplantation,dystrophin expression was observed. Results Comparing to D1-hMDCs,D6-hMDCs and D8-hMDCs showed higher expression of CD56,CD45,CD184,desmin,MHC and dystrophin,while there was no difference between D6-hMDCs and D8-hMDCs. Conclusion D6-hMDCs and D8-hMDCs show similar biological characteristics and belong to MDSCs. They have stronger ability of myogenic differentiation and regeneration than D1-hMDCs.

关 键 词：慢贴壁 肌源性干细胞 成肌分化 MDX 细胞移植 假肥大肌营养不良症 

分 类 号：R746.2[医药卫生—神经病学与精神病学]