静磁场连续曝磁对牙髓干细胞增殖和分化的影响  

Effects of continuous exposure to static magnetic field on the proliferation and differentiation of dental pulp stem cells

作　　者：夏阳[1,2] 陈慧敏 胡姝颖[2] 孙剑飞[1] 郭宇[2] 贾璐[2] XIA Yang;CHEN Huiming;HU Shuying;SUN Jianfei;GUO Yu;JIA Lu(Jiangsu Key Laboratory for Biomaterials and Devices,Southeast University,Nanjing 210009;Jiangsu Key Laboratory of Oral Disease,Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]东南大学江苏省生物材料与器件重点实验室,江苏南京210009 [2]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2020年第2期191-194,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81771044);中国博士后基金(2015M571647);江苏省博士后基金(1402044B);江苏高校优势学科建设工程资助项目(2018-87);江苏省青年医学人才(QNRC2016853);东南大学南京医科大学合作研究项目(2242018K3DN16)。

摘　　要：目的:研究静磁场连续曝磁对牙髓干细胞增殖和分化的影响。方法:搭建静磁场下细胞培养装置,磁通强度(35±5)mT,以常规培养的细胞为对照组。用扫描电镜检测磁场下与常规培养细胞的形态,以CCK-8检测细胞的增殖情况,同时检测细胞的碱性磷酸酶活性,并进行茜素红染色以说明矿化物形成情况。结果:扫描电镜显示,磁性培养的细胞与常规培养的细胞在形态上没有显著差异;磁场下与常规培养下的细胞增殖情况也没有差异;但是磁场下细胞的碱性磷酸酶活性显著增高(P<0.05),且矿化物的形成也显著增多(P<0.05)。结论:静磁场连续曝磁对牙髓干细胞的形态和增殖没有影响,但促进了其成骨方向的分化。Objective:This stady aims to observe the effects of continuous exposure to static magnetic field(SMF)on the proliferation and differentiation of dental pulp stem cells(DPSCs).Methods:The magnetic cell culture set was used with the flux intensity about(35±5)mT.The normal cultured cells were used as control.The morphology of the cells was detected by scanning electron microscopy(SEM).The cell proliferation was detected by CCK-8,and the alkaline phosphatase activity(ALP)was also measured.Alizarin red staining(ARS)was used to detect the mineral synthesis.Results:There were no significant differences in SEM images and cell proliferation between magnetic cultured cells and normal cultured cells,but the ALP activity of magnetic cultured cells was significantly increased(P<0.05),and the mineralization formation of cells under magnetic culture was significantly more than that of normal cultured cells(P<0.05).Conclusion:Continuous exposure to SMF neither changed the cell morphology nor the cell proliferation,but significantly promoted the differentiation of DPSCs.

关 键 词：静磁场 牙髓干细胞 增殖 分化 

分 类 号：R783.1[医药卫生—口腔医学]