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作 者:白俊艳[1,2] 时坤鹏 卢小宁 杨帅 陈梦柯[1] 马永康 巩慧荣 董智豪 庞有志 雷莹[1,2] 曹慧 安肖凯 常世增[1] 侯伟晓 付学言 樊红灯 曹恒 BAI Junyan;SHI Kunpeng;LU Xiaoning;YANG Shuai;CHEN Mengke;MA Yongkang;GONG Huirong;DONG Zhihao;PANG Youzhi;LEI Ying;CAO Hui;AN Xiaokai;CHANG Shizeng;HOU Weixiao;FU Xueyan;FAN Hongdeng;CAO Heng(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023,China;Luoyang Key Laboratory of Animal Genetics and Breeding,Luoyang 471023,China)
机构地区:[1]河南科技大学动物科技学院,河南洛阳471023 [2]洛阳市动物遗传育种重点实验室,河南洛阳471023
出 处:《河南农业科学》2020年第4期147-152,共6页Journal of Henan Agricultural Sciences
基 金:国家自然科学基金项目(31201777);河南省产学研合作项目(152107000095.0)。
摘 要:为了寻找蛋用鹌鹑早期生长性能的分子标记,利用PCR-SSCP检测肌细胞生成素(MyoG)基因5′调控区A和B位点在中国黄羽鹌鹑、北京白羽鹌鹑、朝鲜鹌鹑群体中的多态性,并对MyoG基因5′调控区与鹌鹑的早期生长性能进行了关联性分析。结果表明,MyoG基因5′调控区A位点在蛋用鹌鹑中检测到AA、BB、CC、AB、AC、BC等6种基因型。MyoG基因5′调控区A位点的不同基因型与蛋用鹌鹑的体质量、胫长、胸宽、胸深、胸骨长、体长存在关联性(P<0.05)。MyoG基因5′调控区B位点在蛋用鹌鹑中检测到AA、BB、CC、DD、EE、AB、AC、AD、AE等9种基因型,不同基因型与蛋用鹌鹑的体质量、胫长、胸宽、胸深、胸骨长、体长存在关联性(P<0.05)。综上,MyoG基因5′调控区A、B位点对蛋用鹌鹑的早期生长性能有一定影响,可以作为蛋用鹌鹑早期生长性能的分子标记。In order to find molecular markers for early growth performance of egg quails,PCR-SSCP was used to detect the polymorphism of MyoG gene 5′regulatory region A and B loci in Chinese yellow,Beijing white and Korean quails,and the relationship between the 5′regulatory region of MyoG gene and early growth and development performance of quails was also analyzed.The results showed that 6 genotypes such as AA,BB,CC,AB,AC and BC were detected in the 5′regulatory region A locus of MyoG gene in egg quails.Different genotypes of MyoG gene 5′regulatory region A locus were associated with body weight,tibia length,chest width,chest depth,sternum length,body length of egg quails(P<0.05).Nine genotypes such as AA,BB,CC,DD,EE,AB,AC,AD and AE were detected in egg quails at site B of 5′regulatory region of MyoG gene.Different genotypes were correlated with body weight,tibia length,chest width,chest depth,sternum length and body length of egg quail(P<0.05).In conclusion,the A and B loci in the 5′regulatory region of MyoG gene have some effects on the early growth performance of egg quails.The A and B loci in the 5′regulatory region of MyoG gene can be used as molecular markers for the early growth performance of egg quails.
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