烟草丙酮酸脱羧酶家族生物信学分析  

Bioinformatics Analysis of Tobacco Pyruvate Decarboxylase Family

作　　者：王天龙 梁永标 何俊龙[2] 李江美 李春荣 高文佑 官崇植 苏家恩[5] WANG Tian-long;LIANG Yong-biao;HE Jun-long;LI Jiang-mei;LI Chun-rong;GAO Wen-you;GUAN Chong-zhi;SU Jia-en(Yongping County Branch of Dali Prefecture Tobacco Company,Yongping 672100,PRC;Heqing County Branch of Dali Prefecture Tobacco Company,Heqing 672100,PRC;Jianchuan County Branch of Dali Prefecture Tobacco Company,Jianchuan 671300,PRC;Weishan County Branch of Dali Prefecture Tobacco Company,Weishan 672400,PRC;Dali Prefecture Company of Yunnan Tobacco Company,Dali 671000,PRC)

机构地区：[1]大理州烟草公司永平县分公司,云南永平672100 [2]大理州烟草公司鹤庆县分公司,云南鹤庆672100 [3]大理州烟草公司剑川县分公司,云南剑川671300 [4]大理州烟草公司魏山县分公司,云南魏山672400 [5]云南省烟草公司大理州公司,云南大理671000

出　　处：《湖南农业科学》2020年第3期4-7,共4页Hunan Agricultural Sciences

摘　　要：为研究烟草丙酮酸脱羧酶家族特性,运用ProtScale、CDD、Interpro、Muscle、phyml和TreeDyn等工具分析烟草丙酮酸脱羧酶家族各成员的亲疏水性、结构域和进化树。结果表明:NtPDC_1和NtPDC_2具有亲水性,属于可溶性蛋白,NtPDC_3,NtPDC_4,NtPDC_5和NtPDC_6具有疏水性,为非可溶性蛋白;烟草丙酮酸脱羧酶属于硫胺素焦磷酸(TPP)依赖性酶超家族,各成员均含有3组结构域,分别为N-末端TPP结构域、中心域和C-末端TPP结构域;烟草、拟南芥、水稻、薏苡和玉米的丙酮酸脱羧酶聚类为4组,其中烟草丙酮酸脱羧酶主要聚集在Ⅰ组和Ⅱ组,其进化相对保守。In order to study the characteristics of pyruvate decarboxylase family in tobacco,we used Protscale,CDD,Interpro,Muscle,PhyMl and TreeDyn tools to read the data of water affinity,domain and evolution tree of each member of pyruvate decarboxylase family in tobacco.The results show that NtPDC_1 and NtPDC_2 are hydrophilic and belong to soluble protein.NtPDC_3,NtPDC_4,NtPDC_5 and NtPDC_6 are hydrophobic and insoluble protein.Tobacco pyruvate decarboxylase belongs to the thiamine pyrophosphate(TPP)dependent enzyme superfamily,each member contains three groups of domains:N-terminal TPP domain,central domain and C-terminal TPP domain.Pyruvate decarboxylase clusters of tobacco,Arabidopsis,rice,Coix,maize are divided into four groups,among which tobacco pyruvate decarboxylase mainly gathers in group I and group II,and its evolution is relatively conservative.

关 键 词：烟草 丙酮酸脱羧酶 蛋白特性 聚类分析 生物信息学 

分 类 号：Q557.9[生物学—生物化学]