季也蒙毕赤酵母菌还原Cr(Ⅵ)的特性研究  

Reduction characteristics of Cr(Ⅵ)by Pichia guilliermondii

作　　者：郝孔利 张子辉 王珊珊 张杰[1] HAO Kongli;ZHANG Zihui;WANG Shanshan;ZHANG Jie(Key Laboratory of Saline-Alkali Vegetation Ecology Restoration,Ministry of Education/College of Life Sciences,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北盐碱植被恢复与重建教育部重点实验室/东北林业大学生命科学学院,哈尔滨150040

出　　处：《华中农业大学学报》2020年第2期63-70,共8页Journal of Huazhong Agricultural University

基　　金：黑龙江省自然科学基金联合引导项目(LH2019C005);哈尔滨市科技创新人才项目(2017RALXJ010)。

摘　　要：从喂食聚乙烯塑料印度谷螟(Plodia interpunctella(Hübener))幼虫的肠道液中分离筛选出1株能还原Cr(Ⅵ)的酵母菌ZJH-1,通过形态特征和分子生物学鉴定该菌株为季也蒙毕赤酵母菌(Pichia guilliermondii)。该菌株对Cr(Ⅵ)的最小抑菌浓度(MIC)为17 mmol/L,在含有50 mg/L Cr(Ⅵ)的条件下,对其他重金属的MIC分别为Cu(Ⅱ)13 mmol/L、Pb(Ⅱ)6 mmol/L、Ag(Ⅰ)4 mmol/L、Mn(Ⅱ)16 mmol/L、Ni(Ⅱ)3 mmol/L、Co(Ⅱ)3 mmol/L、Cd(Ⅱ)0.9 mmol/L和Hg(Ⅱ)0.2 mmol/L。以Cr(Ⅵ)还原率为指标,考察了温度、pH、接种量、供电子体以及不同重金属离子对Cr(Ⅵ)还原率的影响,结果表明:在酵母浸出粉胨葡萄糖液体培养基(YPD)中,菌株ZJH-1还原Cr(Ⅵ)的较优pH和温度分别为9.0和40℃;在最适条件下,接种量为8%时,Cr(Ⅵ)的还原效果最佳;当葡萄糖作为供电子体时,48 h内Cr(Ⅵ)的还原率为100%;菌株在含有不同重金属离子的培养液中,除Cu(Ⅱ)、Pb(Ⅱ)和Co(Ⅱ)外,菌株ZJH-1还原Cr(Ⅵ)的能力和生长能力均受到抑制,抑制顺序为Mn(Ⅱ)>Hg(Ⅱ)>Ag(Ⅰ)>Ni(Ⅱ)>Cd(Ⅱ)。对菌株ZJH-1还原Cr(Ⅵ)能力的初步研究结果表明,菌株ZJH-1在Cr(Ⅵ)污染修复中具有良好的应用潜力。A strain of yeast ZJH-1 capable of reducing Cr(Ⅵ)was isolated and screened from the intestinal juice of Plodia interpunctella(Hübener)larvae polyethylene plastic.The results of morphological characteristics and molecular biological identification indicated that the strain is Pichia guilliermondii.The minimum inhibitory concentration(MIC)of Cr(Ⅵ)was 17 mmol/L.Under the condition of 50 mg/L Cr(Ⅵ),the MIC of other heavy metals was Cu(Ⅱ)13 mmol/L,Pb(Ⅱ)6 mmol/L,Ag(Ⅰ)4 mmol/L,Mn(Ⅱ)16 mmol/L,Ni(Ⅱ)3 mmol/L,Co(Ⅱ)3 mmol/L,Cd(Ⅱ)0.9 mmol/L and Hg(Ⅱ)0.2 mmol/L.The effects of temperature,pH,inoculum size,electron donor and different heavy metal ions on Cr(Ⅵ)reduction rate was investigated by Cr(Ⅵ)reduction rate.The results showed that the optimal pH and temperature of strain ZJH-1 for reducing Cr(Ⅵ)were 9.0 and 40°C in yeast leachate glucose liquid medium(YPD).Under optimal conditions,the inoculum was 8%.When Cr(Ⅵ)is the best,the reduction rate of Cr(Ⅵ)is 100%within 48 h when glucose is used as the electron donor.The strain contains Cu(Ⅱ)in the culture solution containing different heavy metal ions.Except for Pb(Ⅱ)and Co(Ⅱ),both the growth ability and ability of strain ZJH-1 to reduce Cr(Ⅵ)were inhibited.The order of inhibition was Mn(Ⅱ)>Hg(Ⅱ)>Ag(Ⅰ)>Ni(Ⅱ))>Cd(Ⅱ).The result of a preliminary study on the ability of strain ZJH-1 to reduce Cr(Ⅵ)showed that strain ZJH-1 has good application potential in the remediation of Cr(Ⅵ)pollution.

关 键 词：季也蒙毕赤酵母菌(Pichia guilliermondii) Cr(Ⅵ) 重金属 污染修复 最小抑菌浓度 铬还原菌 

分 类 号：X172[环境科学与工程—环境科学]