食管康对反流性食管炎模型大鼠呼吸链ComplexⅠ、Ⅳ活性影响的实验研究  

Observation of Shiguankang on Respiratory Chain ComplexⅠandⅣActivities of Reflux Esophagitis Model Rats

作　　者：胡蓆宝[1] 田晶晶 袁红霞 HU Xi-bao;TIAN Jing-jing;YUAN Hong-xia(Department of Spleen and Stomach,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin(300193),China)

机构地区：[1]天津中医药大学第一附属医院脾胃科,天津300193

出　　处：《中国中西医结合外科杂志》2020年第2期243-248,共6页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金项目(81173243)。

摘　　要：目的:观察食管康对反流性食管炎(RE)模型大鼠complexⅠ、Ⅳ活性的影响。方法:将64只健康雄性Wistar大鼠随机分为4组,即正常对照组、模型组、西药治疗组和食管康治疗组,每组16只。采用改良的“食管-十二指肠端侧吻合术”制备大鼠RE模型,分为模型组、食管康组及西药组。从术后第6天开始,正常对照组、模型组给予生理盐水灌胃,药物治疗组分别给予食管康、兰索拉唑+加枸橼酸莫沙必利灌胃,治疗14天后,观察RE模型大鼠食管组织complexⅠ、Ⅳ活性的表达情况。结果:模型组大鼠食管组织complexⅠ、Ⅳ活性较正常组明显降低,差异有统计学意义(P<0.01),食管康组及西药组大鼠食管组织complexⅠ、Ⅳ活性较模型组明显升高,差异有统计学意义(P<0.05),食管康组与西药组的大鼠食管组织complexⅠ、Ⅳ活性表达差异无统计学意义(P>0.05)。结论:食管康提高食管组织中complexⅠ、Ⅳ活性,保持呼吸链完整性,增加线粒体氧利用能力和氧化磷酸化功能,从而改善线粒体膜结构的完整性,起到对RE的治疗作用。Objective To observe the effect of Shiguankang on the complexⅠandⅣactivities of reflux esophagitis(RE)model rats.Methods 64 male Wistar rats were randomly divided into 4 groups:normal control group,model control group,western medicine group and Shiguankang group,which 16 rats in each group.The model group was given the operation of esophagus through improved end-to-side anastomosis of the duodenum.Five days after the operation,the alive rats were randomly divided into model control group,western medicine group and Shiguankang group.From the sixth day after the operation,NS normal saline was given to the normal group and model control group,and Shiguankang was to the Shiguankang group,meanwhile,gavelans oprazole combined with mosapride were to the western medicine control group.Then the activities of complexⅠandⅣwere observed.Results The activities of complexⅠandⅣof the model group were significantly lower than that in the normal group,which the difference was statistically significant(P<0.01).The activities of complexⅠandⅣof the western medicine group and Shiguankang group were higher than those in the model group,which the difference were statistically significant(P<0.05).Compared with the western medicine group,the activities of complexⅠandⅣof Shiguankang group increased unclearly,which the difference was not statistically significant(P>0.05).Conclusion By adjusting Qi movement of spleen and stomach,Shiguankang can increase the activities of complexⅠandⅣof esophageal tissue,maintain the integrity of Mitochondrial Respiratory Chain,increase the mitochondrial oxygen utilization ability and oxidative phosphorylation function.So it can improve the integrity of mitochondrial membrane structure and treat RE.

关 键 词：反流性食管炎 食管康 气机升降 能量代谢 呼吸链复合物Ⅰ 呼吸链复合物Ⅳ 

分 类 号：R573.3[医药卫生—消化系统]