雾化吸入金葡素诱导主动性免疫防护降低兔金黄色葡萄球菌的感染效应  

Aerosol inhalation of staphylococcal enterotoxin for inducing active immune protection reduces infection effect of Staphylococcus aureus in rabbits

作　　者：简黎[1] 叶永斌[1] 郭子文[1] 张农惠[1] 李宏伟 邱大发[1] 许晓军[1] JIAN Li;YE Yongbin;GUO Ziwen;ZHANG Nonghui;LI Hongwei;QIU Dafa;XU Xiaojun(Department of Hematology,Zhongshan Hospital of Sun Yat-Sen University&People’s Hospital of Zhongshan,Zhongshan,Guangdong 528403,China)

机构地区：[1]中山市人民医院血液内科,广东中山528403

出　　处：《中国微生态学杂志》2020年第3期260-263,共4页Chinese Journal of Microecology

基　　金：中山市科技计划(2014A1FC037);中山市科技计划(2016B1023);广东省医学科学技术研究基金(A2016043)。

摘　　要：目的探索雾化吸入金葡素(staphylococcin)是否诱导主动性免疫防护以降低兔金黄色葡萄球菌(Staphylococcus aureus)的感染效应。方法将金黄色葡萄球菌感染的新西兰大白兔模型30只(雌雄各半,平均体质量2.45 kg)随机分为对照组、雾化组,每组15只。对照组采用生理盐水进行雾化吸入,雾化组采用生理盐水+金葡素雾化吸入,每天1次,共20 d。ELISA法检测各组兔模型采用干预因素处理前后血清IgG抗体滴度,碱性磷酸酶-抗碱性磷酸酶桥联酶染色法(APAAP法)检测T细胞亚群CD4^+、CD8^+T淋巴细胞比例和CD4^+/CD8^+比值的变化。结果雾化组处理后兔血清IgG抗体滴度显著高于对照组,差异有统计学意义(χ^2=18.9451,P<0.01)。同时,雾化组CD4^+T淋巴细胞比例显著高于对照组(t=2.1340,P<0.01),CD4^+/CD8^+比值也明显高于对照组(t=5.2983,P<0.05)。结论雾化吸入金葡素能有效诱导金黄色葡萄球菌感染的兔产生主动性免疫防护,降低金黄色葡萄球菌感染的效应。Objective To explore whether aerosol inhalation of staphylococcal enterotoxin induces active immune protection to reduce the infection effect of Staphylococcus aureus in rabbits. Methods A total of 30 New Zealand white rabbit models(half male and half female, with average body weight of 2.45 kg) infected with Staphylococcus aureus were randomly divided into control group or atomization group. The control group was treated with normal saline via aerosol inhalation, while the atomization group was given inhalation of normal saline and staphylococcal enterotoxin, once a day for 20 days. ELISA was used to detect serum IgG antibody titer before and after the intervention in each group. Alkaline Phosphatase Anti Alkaline Phosphatase(APAAP) staining method was adopted to detect the changes of T cell subset CD4^+, CD8^+T lymphocyte ratio and CD4^+/CD8^+ ratio. Results The serum IgG antibody titer after treatment in the atomization group was significantly higher than that in the control group, with significant difference(χ^2=18.945 1,P<0.01). The proportion of CD4^+T lymphocytes in the atomization group was significantly higher than that in the control group(t=2.134 0,P<0.01), so was the CD4^+/CD8^+ ratio(t=5.298 3,P<0.05). Conclusion Aerosol inhalation of staphylococcal enterotoxin can effectively induce active immune protection in rabbits infected with Staphylococcus aureus, thereby reducing the effect of Staphylococcus aureus infection.

关 键 词：金葡素 金黄色葡萄球菌 免疫防护 

分 类 号：R-33[医药卫生] R392.5