Global Co-transcriptional Splicing in Arabidopsis and the Correlation with Splicing Regulation in Mature RNAs  

作　　者：Shaofang Li Yuan Wang Yonghui Zhao Xinjie Zhao Xuemei Chen Zhizhong Gong 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100193,China [2]Joint Laboratory for International Cooperation in Crop Molecular Breeding,China Agricultural University,Beijing 100193,China [3]Department of Botany and Plant Sciences,Institute of Integrative Genome Biology,University of California,Riverside,CA 92521,USA [4]Gua ngdong Provincial Key Laboratory for Plant Epige netics,Longhua Institute of Innovative Biotech no logy,College of Life Sciences and Oceanography,Shenzhe n University,Shenzhen 518060,China [5]Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China [6]Plant Phenomics Research Center,Nanjing Agricultural University,Nanjing 21001&China

出　　处：《Molecular Plant》2020年第2期266-277,共12页分子植物（英文版）

基　　金：supported by the Fundamental Research Funds for the Central Universities,China and National Science Foundation of China(91740202).Y.W.was supported by a fellowship from Shenzhen Un iversity.

摘　　要：RNA splicing and spliceosome assembly in eukaryotes occur mainly during transcription.However,co-transcriptional splicing has not yet been explored in plants.Here,we built transcriptomes of nascent chromatin RNAs in Arabidopsis thaliana and showed that nearly all introns undergo co-transcriptional splicing,which occurs with higher efficiency for introns in protein-coding genes than for those in noncoding RNAs.Total intron number and intron position are two predominant features that correlate with co-transcriptional splicing efficiency,and introns with alternative 5′or 3′splice sites are less efficiently spliced.Furthermore,we found that mutations in genes encoding trans-acting proteins lead to more introns with increased splicing defects in nascent RNAs than in mature RNAs,and that introns with increased splicing defects in mature RNAs are inefficiently spliced at the co-transcriptional level.Collectively,our results not only uncovered widespread co-transcriptional splicing in Arabidopsis but also identified features that may affect or be affected by co-transcriptional splicing efficiency.

关 键 词：CHROMATIN co-transcriptional SPLICING frans-acting proteins ARABIDOPSIS 

分 类 号：Q943.2[生物学—植物学]