γ-Adducin减轻Aβ1-42诱导的神经元突起损伤  

γ-Adducin alleviated neurite damage induced by Aβ1-42

作　　者：熊敏 孟兰霞 何名扬 张星雨 戴莉君 潘丽娜 张振涛[1] Xiong Min;Meng Lanxia;He Mingyang(Department of Neurology,Renmin Hospital of Wuhan University,Wuhan 430060)

机构地区：[1]武汉大学人民医院神经内科,430060

出　　处：《卒中与神经疾病》2020年第1期14-18,23,共6页Stroke and Nervous Diseases

基　　金：国家自然科学基金(No.81822016,81771382,81571249)。

摘　　要：目的探讨γ-Adducin蛋白是否可减轻Aβ1-42纤维诱导的神经元突起损伤。方法实验一:培养原代神经元,用10μmol/L Aβ1-42纤维处理神经元24 h,采用神经元特异性标记物MAP2检测神经元突起损伤,用TUNEL染色检测神经元凋亡;实验二:分别用对照病毒和表达γ-Adducin的腺相关病毒感染神经元,蛋白表达5 d后再分别加入Aβ1-42纤维处理,用免疫荧光染色法观察γ-Adducin蛋白过表达对Aβ1-42纤维引起的神经元突起损伤的影响,用Dil染色检测神经元树突棘密度的变化。结果 Aβ1-42纤维对神经元的突起具有损伤作用,并且引起神经元凋亡;过表达γ-Adducin蛋白对Aβ1-42纤维引起的神经元突起和树突棘损伤均有保护作用。结论γ-Adducin蛋白可减轻Aβ1-42纤维导致的神经元突起损伤,它可能是1个新的阿尔茨海默病治疗靶点。Objective To explore whether γ-Adducin alleviated neurite damage induced by Aβ1-42 fibrils in neurons.Methods Experiment #1: Primary cultured neurons were exposed to Aβ1-42 fibrils at a concentration of 10μmol/L for 24 hours, neurite change was measured after staining with neuronal marker microtubule associated protein 2(MAP2),TUNEL staining was performed to detect apoptosis. Experiment #2: Primary neurons were pre-treated with adeno-associated virus encoding control or γ-Adducin, respectively,5 days after virus infection, Aβ1-42 fibrils(10μmol/L) were added into both groups, Immunofluorescence staining was used to investigate the effect of γ-Adducin on neurite damage induced by Aβ1-42 fibrils, Dil staining was used to detect the changes of dendritic spines density in primary neurons.Results Aβ1-42 fibrils induced damage of neurites and caused apoptosis in primary neurons. Overexpression of γ-Adducin protected neurites from damage induced by Aβ1-42 fibrils.Conclusion It was found that γ-Adducin alleviated neuronal neurite damage induced by Aβ1-42 fibrils, which indicated that γ-Adducin might be a novel therapeutic target for Alzheimer’s disease.

关 键 词：γ-Adducin 阿尔茨海默病 神经元 突起 

分 类 号：R742[医药卫生—神经病学与精神病学]