血管内皮生长因子基因修饰诱导多能干细胞的生物学特性的研究  

Biological characteristics of the induced pluripotent stem cells modified by vascular endothelial growth factor gene

作　　者：高冠群[1] 方婧[1] 王岩[1] 张卓伯[1] 邵海艳 Gao Guanqun;Fang Jing;Wang Yan(Department of Neurology,The Fourth affiliated Hospital of Harbin Medical University,Harbin 150001)

机构地区：[1]哈尔滨医科大学附属第四医院神经内科,150001 [2]哈尔滨市第一医院神经内科

出　　处：《卒中与神经疾病》2020年第1期24-27,32,共5页Stroke and Nervous Diseases

基　　金：黑龙江省教育厅资助项目(编号为12541539)。

摘　　要：目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)基因修饰诱导多能干细胞(induced pluripotent stem cells,iPS)的生物学特性。方法将培养好的iPS细胞分为3组,即N0(纯iPS培养)组、N1(空腺病毒载体转染iPS)组、N2(转染VEGF基因的iPS)组;3组细胞分别进行传代培养,并采用MTT方法检测转染VEGF基因对细胞增殖的影响;采用酶联免疫(ELISA)方法检测细胞培养上清液中VEGF的表达水平。结果转染VEGF基因对iPS细胞增殖没有明显影响;N2组iPS细胞分泌的VEGF蛋白表达水平较N1组显著增加(P<0.05)。结论经VEGF基因转染的iPS可正常存活生长,并可稳定表达该基因。Objective To explore biological characteristics of the induced pluripotent stem cells(iPS) modified by vascular endothelial growth factor(VEGF) gene.Methods The cultured iPS cells were divided into three groups:N0(pure iPS culture) group,N1(empty adenovirus vector transfection iPS)group,N2(iPS group transfected with VEGF gene) group.Three groups of cells were subcultured respectively, and MTT method was determined to detect transfection VEGF gene effects on cell proliferation. The enzyme-linked immune(ELISA) method was used to detect the expression level of VEGF in cell culture supernatant on content. Results The cell proliferation of the transferred iPS was similar to those of the N0(pure iPS culture) group, N1(empty adenovirus vector transfection iPS)group. The level of VEGF protein in supernatant after VEGF gene transfection was significantly higher than that of the N1(empty adenovirus vector transfection iPS) group(P<0.05).Conclusion VEGF gene transfection iPS could grow normally and stably express the gene.

关 键 词：诱导多能干细胞 血管内皮生长因子 转染 基因表达 

分 类 号：R741[医药卫生—神经病学与精神病学]