宫颈癌合并HPV感染患者HPV不同亚型分布及脆性组氨酸三联体蛋白表达  

Subtypes of HPV and expression of fragile histidine triad protein in cervical cancer patients complicated with HPV infection

作　　者：祁美霞[1] 靳荣[1] 卢慧[1] 李宏[1] 吴晓蕊 尚欣荣[2] QI Mei-xia;JIN Rong;LU Hui;LI Hong;WU Xiao-rui;SHANG Xin-rong(Tianjin Fifth Central Hospital,Tianjin 300450,China)

机构地区：[1]天津市第五中心医院妇产科,天津300450 [2]首都医科大学附属北京同仁医院检验科,北京100730

出　　处：《中华医院感染学杂志》2020年第5期734-737,共4页Chinese Journal of Nosocomiology

基　　金：天津市滨海新区卫生局科技基金资助项目(2014BWKY015)。

摘　　要：目的探究宫颈癌合并人乳头瘤病毒(HPV)感染患者HPV不同亚型分布及脆性组氨酸三联体(FHIT)蛋白表达情况.方法选取2015年6月-2018年6月于首都医科大学附属北京同仁医院行手术切除宫颈癌患者45例作为宫颈癌组,同期收治子宫肌瘤行子宫全切、宫颈健康患者30例为对照组.检测HPV基因分型及FHIT蛋白表达水平;比较宫颈癌组织和正常组织聚合酶链反应产物电泳图谱,检测FHIT外显子D3S1234和D3S1300微卫星多态标记杂合性丢失(LOH)和微卫星不稳定性(MI).结果45例宫颈癌检出13种HPV亚型,以高危型感染为主.宫颈癌HPV亚型感染检出单一亚型感染31例、双重感染8例、多重感染6例.宫颈癌组FHIT表达阳性率28.89%低于对照组(P<0.05);FHIT表达与宫颈癌病理分级和淋巴结转移关系密切(P<0.05);宫颈癌LOH或MSI出现频率80.00%,外显子D3S1234 MSI频率24.44%、LOH频率51.11%、D3S1300 MSI频率22.22%、LOH频率68.89%,均出现MSI 2例、LOH 13例.结论高危型HPV感染与宫颈癌发生发展密切相关,FHIT基因MSI、LOH及蛋白表达水平与宫颈癌关系密切,为宫颈癌筛查诊断提供参考价值.OBJECTIVE To explore the distribution of subtypes of human papillomavirus(HPV)and expression of fragile histidine triad(FHIT)protein in cervical cancer patients complicated with HPV infection.METHODS Totally 45patients with cervical cancer who received surgical resection in Beijing Tongren Hospital Affiliated to Capital Medical University from Jun 2015to Jun 2018were assigned as the cervical cancer group,meanwhile,30 without cervical caner who underwent hysterectomy for uterine fibroids were set as the control group.The genotypes of HPV and FHIT protein level were detected,the electrophoretograms of PCR products were observed and compared between the cervical cancer tissues and the normal tissues,and the loss of heterozygosity(LOH)and microsatellite instability(MSI)of FHIT exons D3S1234and D3S1300were detected.RESULTS Totally 13subtypes of HPV were detected in the 45cervical cancer patients,and the high-risk subtype was dominant.Among the cervical cancer patients who had the infections with HPV subtypes,31had single subtype infection,8had double infections,and 6had multiple infections.The positive rate of expression of FHIT of the cervical cancer group was 28.89%,significantly lower than that of the control group(P<0.05).The expression of FHIT was closely associated with pathological grade and lymph node metastasis of cervical cancer(P<0.05).The frequency of occurrence of LOH or MSI was 80.00%in the cervical cancer patients,the MSI frequency of exon D3S1234was 24.44%,the LOH frequency was 51.11%;the MSI frequency of D3S1300was 22.22%,and the LOH frequency was 68.89%.There were 2cases of MSI and 13cases of LOH in the above two seats.CONCLUSIONThe high-risk HPV infection is closely related to the occurrence and development of cervical cancer.The MSI,LOH and protein expression levels of FHIT gene are closely associated with the cervical cancer,which have reference value for screening and diagnosis of cervical cancer.

关 键 词：宫颈癌 人乳头瘤病毒 人乳头瘤病毒亚型 脆性三联组氨酸 微卫星多态标记 

分 类 号：R737.33[医药卫生—肿瘤]