骨髓单个核细胞移植对大鼠局灶性脑缺血后神经功能恢复的影响  

Effects of bone marrow mononuclear cell transplantation on the recovery of nerve function after focal cerebral ischemia and reperfusion

作　　者：刘建锋 胡亚梅 李刚 张钱林 张杰文 Liu Jianfeng;Hu Yamei;Li Gang;Zhang Qianlin;Zhang Jiewert(Department of Neurology,People's Hospital of Zhengzhou University,Henan Provincial People's Hospital,Zhengzhou 450003,China)

机构地区：[1]郑州大学人民医院(河南省人民医院)神经内科,郑州450003

出　　处：《中华物理医学与康复杂志》2020年第1期2-7,共6页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：河南省科技厅重点科技攻关计划项目(122102310094)。

摘　　要：目的:观察骨髓单个核细胞(BMMCs)移植对大鼠局灶性脑缺血后右侧额叶缺血灶周围区胶质纤维酸性蛋白(GFAP)及Nogo-A表达的影响,并探讨其促进神经功能恢复的可能机制。方法:从SD大鼠骨髓中分离BMMCs,采用线栓法制作大鼠右侧大脑中动脉栓塞(MCAO)模型,于缺血再灌注24 h后根据Longa评分选择入组模型,采用随机数字表法将48只MCAO大鼠分为模型组及观察组,每组再细分为7 d、14 d及21 d共3个亚组。将BMMCs缓慢注入观察组缺血侧纹状体内,模型组则注入等体积磷酸盐缓冲液(PBS)。各组大鼠分别于干预后7 d、14 d及21 d时再次进行Longa评分,随后麻醉处死取脑,采用免疫组织化学染色观察各亚组大鼠右侧额叶缺血灶周围脑区GFAP及Nogo-A表达。结果:缺血再灌注21 d时,发现观察组大鼠肢体神经功能缺损评分低于模型组(P<0.05);观察组大鼠在再灌注14 d及21 d时其GFAP表达数量均较模型组增多[(37.62±2.45)vs(27.62±1.69),(38.00±1.85)vs(27.25±1.83),P<0.05],而Nogo-A表达数量均较模型组降低[(28.88±2.64)vs(32.50±1.60),(23.87±2.36)vs(32.00±1.85),P<0.05];观察组大鼠在再灌注21 d时其Nogo-A表达量明显低于再灌注14 d时水平[(23.87±2.36)vs(28.88±2.64),P<0.05]。结论:BMMCs移植能促进局灶性脑缺血大鼠受损神经功能改善,其作用机制可能与促进缺血灶周围脑组织GFAP表达及抑制Nogo-A表达有关。Objective To determine the effect of transplanting bone marrow mononuclear cells(BMMCs)on the expression of glial fibrillary acidic protein(GFAP)and Nogo-A around the ischemic foci after focal cerebral ischemia and reperfusion,and to study any role of BMMCs in nerve function recovery.Methods BMMCs were isolated from the bone marrow of Sprague-Dawley rats.Cerebral ischemia and reperfusion was performed using a nylon thread to occlude the right middle cerebral artery for 2h followed by 24h of reperfusion.The qualified models were selected according to the Longa scale.The 48 models selected were randomly divided into a model group and an observation group,each of 24.Each group was further divided into 7d,14d and 21d subgroups.100μl of BMMCs(5×106/ml)were slowly injected into the ischemic lateral striata of the observation group.The rats in the model group were similarly injected,but with buffered saline solution.The rats were evaluated using the Longa scale after 7d,14d and 21d.The rats were then sacrificed and the brain was resected.Immunohistochemical assays quantified the expression of GFAP and Nogo-A around the ischemic foci.Results Compared with the model group,the rats in the observation group showed less neurological deficit on the 21st day,significantly greater expression of GFAP and significantly less Nogo-A expression on days 14 and 21.Nogo-A expression on the 21st day was also significantly lower than on the 14th day in the observation group.Conclusion BMMC transplantation can promote recovery from nerve damage after focal cerebral ischemia,which is probably related to enhanced expression of GFAP and restrained expression of Nogo-A in the brain tissues surrounding ischemic lesions.

关 键 词：骨髓单个核细胞 脑缺血 胶质纤维酸性蛋白 NOGO-A 

分 类 号：R74[医药卫生—神经病学与精神病学]