结直肠癌中HIF-1α、CD147和MMP-9表达与临床病理特征的关系  

Correlation between Expression of HIF-1α,CD147 and MMP-9 and Clinicopathological Features in Colorectal Cancer

作　　者：朱长龙 王晓琼[2] 何常 ZHU Changlong;WANG Xiaoqiong;HE Chang(College of Clinical Medicine,Guizhou Medical University,Guiyang 550025,Guizhou,China;Department of Fundametal Medicine,Guiyang Nursing Vocational College,Guiyang 550081,Guizhou,China)

机构地区：[1]贵州医科大学临床医学院,贵州贵阳550025 [2]贵阳护理职业学院基础医学部,贵州贵阳550081

出　　处：《贵州医科大学学报》2020年第4期466-471,共6页Journal of Guizhou Medical University

基　　金：贵州省科学技术基金项目[黔科合J字(2010)2158]。

摘　　要：目的:检测结直肠癌组织中缺氧诱导因子-1(HIF-1)、细胞外基质金属蛋白酶诱导剂(CD147)和基质金属蛋白酶(MMP)蛋白表达,探讨3项指标与结直肠癌临床病理特征的关系。方法:37例未经新辅助化疗结直肠腺癌患者切除的肿瘤组织作为实验组,记录患者的性别、年龄,收集肿瘤的病理分级、大小、部位、浸润深度、脉管侵犯、神经侵犯、淋巴结转移及TNM分期等临床病理参数,同时选择这37例患者结直肠癌切除组织旁的正常(癌旁)组织作为对照组;应用免疫组化法标记HIF-1α、CD147及MMP-9蛋白的表达,分析3种蛋白表达与肿瘤大小、淋巴结转移等临床病理特征的关系及3种蛋白之间的关系。结果:实验组HIF-1α、CD147及MMP-9蛋白表达明显高于对照,HIF-1α和CD147蛋白表达与肿瘤大小差异有统计学意义(P<0.05);HIF-1α、CD147和MMP-9蛋白表达与淋巴结转移有关,差异有统计学意义(P<0.05);结直肠癌肿瘤组织中,HIF-1α、CD147和MMP-9蛋白表达呈正相关(P<0.05)。结论:结直肠癌中HIF-1α、CD147和MMP-9蛋白过度表达可能参与了肿瘤发展及转移,可作为评估结直肠癌生物学行为的指标。Objective:To detect the expression of HIF-1α,CD147,MMP-9 and MMP in colorectal cancer tissues,and to explore the relationship between the three indicators and the clinicopathological features of colorectal cancer.Methods:The tumor tissue of 37 patients with colorectal adenocarcinoma without neoadjuvant chemotherapy was as experimental group.The patient's sex,age,and collected clinicopathological parameters such as pathological grade,size,location,depth of invasion,vascular invasion,nerve invasion,lymph node metastasis and TNM stage of the tumor were recorded.The normal(paracancerous)tissue adjacent to the resection of colorectal cancer was also selected as the control group in these 37 patients.The expression of HIF-1α,CD147 and MMP-9 proteins was labeled by immunohistochemical method,and the relationship between the expression of three proteins and the clinicopathological features of tumor size and lymph node metastasis was analyzed.Results:The expression of HIF-1α,CD147 and MMP-9 protein in the experimental group was significantly higher than that in the control group.The difference between HIF-1αand CD147 protein expression and tumor size was statistically significant(P<0.05).The expression of HIF-1α,CD147 and MMP-9 protein was related to lymph node metastasis,and the difference was statistically significant(P<0.05).HIF-1α,CD147 and MMP-9 protein expression were positively correlated in colorectal cancer tumor tissues(P<0.05).Conclusion:Overexpression of HIF-1α,CD147 and MMP-9 proteins in colorectal cancer is involved in tumor development and metastasis,and it can be used as an indicator to assess biological behavior in colorectal cancer.

关 键 词：结直肠肿瘤 基质金属蛋白酶类 病理过程 缺氧诱导因子-1 蛋白 表达 

分 类 号：R735.34[医药卫生—肿瘤]