μ阿片受体通过调控Smad2抑制乳腺癌细胞迁移侵袭  

Mu Opioid Receptor Suppresses Migrationand Invasion of MDA-MB-231 through Regulating Smad2

作　　者：林俊杰 谭梓聪 杨浩杰 曹铭辉[1] LIN Jun?jie;TANG Zi?cong;YANG Hao?jie;CAO Ming?hui(Department of Anesthesiology,Sun Yat?sen Memorial Hospital,Sun Yat?sen University,Guangzhou,510120)

机构地区：[1]中山大学孙逸仙纪念医院麻醉科,510120

出　　处：《岭南急诊医学杂志》2020年第1期43-47,共5页Lingnan Journal of Emergency Medicine

基　　金：国家自然科学基金资助项目(81471352)。

摘　　要：目的:研究μ阿片受体(MOR)对乳腺癌细胞侵袭及迁移的影响及其机制。方法:Western blot(WB)及RT?qPCR检测乳腺癌细胞系MCF?7、BT?549、SKBR3、MDA?MB?231、BT?474、T47D中MOR的表达水平。在MDA?MB?231细胞中,慢病毒转染过表达MOR。用划痕实验、transwell实验以及WB实验来研究MOR表达量改变对乳腺癌MDA?MB?231细胞侵袭、迁移的影响及可能的机制。结果:WB及RT?qPCR结果示,MOR在MDA?MB?231中的表达量明显低于MCF?7、BT?549、SKBR3、BT?474、T47D。划痕实验及transwell实验示,过表达MOR后MDA?MB?231细胞愈合、迁移及侵袭能力明显减弱。WB结果示,与对照组相比,过表达组的Smad2、pSmad2、MMP9、MMP2、N?cadherin表达量下调。结论:与MCF?7等低转移潜能细胞相比,MOR在高转移潜能乳腺癌细胞系MDA?MB?231中表达量低。过表达MOR后,MDA?MB?231细胞迁移、侵袭能力减弱。WB结果提示MOR对MDA?MB?231细胞迁移侵袭的影响可能与Smad2及其下游蛋白的表达下调有关。Objective:To investigate the effects of the mu opioid receptor(MOR)on breast cancer cells and the underlying molecular mechanisms.Methods:The expression of MOR in breast cancer cells was measured by western blotting assay(WB)and real?time quantitative polymerase chain reaction(RT?qPCR).To overexpress MOR in MDA?MB?231 used lentiviral Vector.Wound healing and transwell assays were used to detect the effects of MOR on the migratory and invasive ability of MDA?MB?231.And we carried out WB to detect the variations in expression of downstream pro?teins due to overexpression of MOR.Results:The expression of MOR in MDA?MB?231 was lower compared with other breast cells.Invasion and metastasis were inhibited when MOR was upregulated.According to the results of WB,we knew the overexpression of MOR could reduce the expression of Smad2,pSmad2,MMP9,MMP2 and N?cadherin.Con?clusion:The level of MOR is lower in breast cell with high metastatic potential.The overexpression of MOR could partly inhibit invasion and metastasis of breast cancer by regulating the expression of Smad2 and pSmad2.

关 键 词：Μ阿片受体 SMAD2 乳腺癌 侵袭 迁移 

分 类 号：R737.9[医药卫生—肿瘤]