DNA定量分析联合hMSH6检测在宫颈癌筛查中的意义  

The significance of DNA quantitative analysis combined with hMSH6 in cervical cancer screening

作　　者：王娜娜[1] 张骞[1] 高淑红[2] 吴淑华[1] 王玉堂 WANG Nana;ZHANG Qian;GAO Shuhong;WU Shuhua;WANG Yutang(Department of Pathology,Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China;Department of Gynecology,Binzhou Medical University Hospital;School of Clinical Medicine,Binzhou Medical University)

机构地区：[1]滨州医学院附属医院病理科,山东滨州256603 [2]滨州医学院附属医院妇产科 [3]滨州医学院临床医学院

出　　处：《滨州医学院学报》2020年第2期87-91,共5页Journal of Binzhou Medical University

基　　金：山东省医药卫生科技发展计划(2017WS152,2016WS0010);滨州市科技发展计划(2015ZCZ0324);大学生创新训练项目(S201910440012)。

摘　　要：目的探讨DNA异倍体分析与hMSH6及HPV在宫颈癌变过程中的相关性及临床意义。方法采用高危型HPV-DNA检测及免疫组化hMSH6染色,分析不同病变宫颈组织中hMSH6表达、HPV感染情况及相关性。结果以DNA异倍体出现和中量DNA异倍体出现为活检标准,宫颈病变的特异性和敏感性分别为25.86%和77.59%。提示中量以上DNA异倍体出现对宫颈病变筛查具有较高的诊断价值。多量、中量异倍体组hMSH6阳性率分别为75%和85.71%,与对照组间比较,P<0.05。多量、中量DNA异倍体组HPV感染率分别为92.86%和76.19%,与对照组间比较,P<0.05。HPV感染多量倍体异常组hMSH6阳性率为90.38%,与无HPV感染多量倍体异常组MSH6阳性率25%比较,P<0.05。结论HPV联合hMSH6检测是针对病因的检测,敏感性高。HPV感染导致hMSH6蛋白高表达,有望为宫颈癌的防治提供参考依据。Objective To investigate the correlation and clinical significance of DNA ploidy analysis with hMSH6 and HPV in cervical cancer.Methods High risk HPV-DNA detection and immunohistochemical hMSH6 staining were used to analyze the expression of hMSH6,HPV infection and correlation in cervical tissues with different lesions.Results The specificity of cervical lesions was 25.86%and 77.59%,respectively,based on the presence of DNA aneuploidy and moderate amount of DNA aneuploidy.It suggests that the presence of more than moderate amount of DNA aneuploidy has a higher diagnostic value for cervical lesions screening.The positive rates of hMSH6 were 75%and 85.71%in the multidose and medium dose alloploid groups,which had statistically significant differences from the control group(P<0.05).The infection rate of HPV was 92.86%and 76.19%in the multi-dose and medium-dose DNA aneuploidy groups,which showing statistically significant differences from the control group(P<0.05).The MSH6 positive rate of the multiploidy abnormal HPV infection group was 90.38%,which was different from the MSH6 positive rate of the multiploidy abnormal HPV infection group 25%(P<0.05)Conclusion HPV combined with hMSH6 test is the detection of etiology,with high sensitivity.The high expression of hMSH6 protein caused by HPV infection is expected to provide a reference for the prevention and treatment of cervical cancer.

关 键 词：宫颈病变 DNA倍体分析 HMSH6 HPV感染 

分 类 号：R737.33[医药卫生—肿瘤]