子宫颈癌中miR-153和Survivin的表达及其临床意义  

Expression of miR-153 and Survivin in cervical cancer patients and its clinical significance

作　　者：周成友 杜阳 赵静[1] 李良 ZHOU Cheng-you;DU Yang;ZHAO Jing;LI Liang(Department of Pathology, Southeast University Affiliated Zhongda Hospital Jiangbei District/Nanjing Dachang Hospital, Nanjing 210044, Jiasngsu, China)

机构地区：[1]东南大学附属中大医院江北院区/南京市大厂医院病理科,南京210044

出　　处：《临床与实验病理学杂志》2020年第3期311-315,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨miR-153和Survivin在子宫颈癌组织中的表达及其对子宫颈癌的临床意义。方法收集50例子宫颈癌和40例正常子宫颈组织,采用RT-PCR法和免疫组化SP法检测miR-153和Survivin在子宫颈癌和正常子宫颈组织中的表达,分析两者表达与子宫颈癌临床病理特征的关系及对患者预后的意义。结果miR-153在子宫颈癌组织中的相对表达量低于正常子宫颈组织,Survivin在子宫颈癌组织中的相对表达量高于正常子宫颈组织,差异均有统计学意义(t=16.333,t=6.407,P<0.01)。通过散点图及相关性分析,在子宫颈癌组织中miR-153和Survivin相对表达量呈负相关,差异有统计学意义(P<0.01);miR-153、Survivin表达与患者年龄、肿瘤大小、TNM分期均无关(P>0.05),与淋巴结转移明显相关(χ^2=6.455,χ^2=5.937,P<0.05)。通过散点图及相关性分析,淋巴结转移患者的淋巴结受侵数目与miR-153表达量呈负相关,与Survivin表达量呈正相关,差异均有统计学意义(P<0.01)。预后生存分析发现,miR-153高表达患者生存率高于低表达患者,Survivin高表达患者生存率低于低表达患者,差异均有统计学意义(χ^2=19.390,χ^2=27.921,P<0.01)。结论miR-153在子宫颈癌组织中低表达,与Survivin在子宫颈癌组织中表达水平呈负相关,可能与子宫颈癌的增殖、侵袭转移有关。Purpose To investigate the expression of miR-153 and Survivin in cervical cancer and its clinical significance for cervical cancer.Methods 50 specimens of cervical cancer and 40 specimens of normal cervical tissue were selected.The expression of miR-153 and Survivin in cervical cancer tissues and normal cervical tissues was detected by RT-PCR and immunohistochemical SP method,respectively.The relationship between miR-153,Survivin expression and clinicopathological parameters of patients with cervical cancer and its significance for prognosis was also analysed.Results The relative expression of miR-153 in cervical cancer tissues was lower than that in normal cervical tissues,and the relative expression of Survivin in cervical cancer tissues was higher than that in normal cervical tissues(t=16.333,t=6.407,P<0.01).By using scatter plot and correlation analysis,there was a negative correlation between the relative expression of miR-153 and Survivin in cervical cancer tissues,and the difference was statistical significant(P<0.01).In cervical cancer patients,the expression of miR-153 and Survivin had no significant relationship with age,tumor size and TNM stage(P>0.05),but was significantly correlated with lymph node metastasis(χ^2=6.455,χ^2=5.937,P<0.05).By using scatter plot and correlation analysis,there was a negative correlation between the number of lymph node involvement and the expression of miR-153,and a positive correlation between the number of lymph node involvement and the expression of Survivin(P<0.01).The prognosis survival analysis of patients with cervical cancer found that the survival rate of patients with high expression of miRNA-153 was higher than that of patients with low expression of miR-153,and the survival rate of patients with high expression of Survivin was lower than that of patients with low expression of Survivin(χ^2=19.390,χ^2=27.921,P<0.01).Conclusion The low expression miR-153 in cervical cancer is negatively correlated with the expression level of Survivin in cervical cancer tiss

关 键 词：子宫颈肿瘤 miR-153 SURVIVIN 免疫组织化学 实时定量荧光PCR 

分 类 号：R737.33[医药卫生—肿瘤]