GATA4通过p38MAPK信号通路调控牙周膜干细胞成骨分化  

GATA4 regulates osteogenic differentiation of human periodontal ligament stem cells via p38MAPK signaling pathway

作　　者：赵西博 冯保静[1] 王国庆 袁清敏 ZHAO Xi-bo;FENG Bao-jing;WANG Guo-qing;YUAN Qing-min(Dental Implantology,the Six-People’s Hospital of Anyang City,Henan 455000,China)

机构地区：[1]安阳市第六人民医院种植科,河南455000

出　　处：《口腔颌面修复学杂志》2019年第6期326-331,共6页Chinese Journal of Prosthodontics

基　　金：安阳市科技计划项目(项目编号:2016-34)。

摘　　要：目的:体外研究GATA4(GATA binding protein 4,GATA4)对人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)成骨分化能力的影响及调控机制。方法:分离培养人牙周膜干细胞,检测GATA4在牙周膜干细胞成骨分化过程中的表达变化;在牙周膜干细胞中沉默或过表达GATA4,qPCR检测GATA4和成骨相关基因mRNA水平的表达;Western blot检测p38MAPK信号通路和GATA4蛋白水平的表达,茜素红染色观察细胞成骨分化能力的变化;检测p38抑制剂(SB203580)对转染GATA4后细胞成骨分化能力的影响。结果:成骨诱导后牙周膜干细胞中GATA4的表达水平显著上调,GATA4过表达可明显促进hPDLSCs成骨基因的表达和钙化结节的形成,而抑制GATA4的表达,则结果相反;GATA4过表达可增强p38 MAPK信号通路的表达,进一步实验结果表明SB203580可逆转过表达GATA4对牙周膜干细胞成骨分化的促进作用。结论:GATA4通过p38 MAPK信号通路促进牙周膜干细胞成骨向分化,提示GATA4在hPDLSCs组织工程损伤修复过程中发挥着重要作用。Objective:To investigate regulation of GATA4 on the osteogenic differentiation capacity of human periodontal ligament stem cell(hDPSCs)in vitro.Methods:The expression of GATA4 were detected in hPDLSCs under conduction of bone induction.Periodontal ligament stem cells were isolated from human healthy periodontal ligament tissue and infected with GATA4.Then the osteogenic differentiation capacity was evaluated by alizarin red assay.The expression of GATA4 were detected by q-RCR andWestern blot.p38 MAPK signaling pathway was detected after infecting with GATA4.Then the osteogenic differentiation capacity was evaluated after utilized with SB203590.Results:The expression of GATA4 were significant up-regulated under the conduction of bone induction.The osteogenic differentiation capacity of hPDLSCs were induced after overexpression of GATA4,and vice versa.Overexpression of GATA4 increased the expression of p38 MAPK signaling.In addition,SB203590 was able to reverse the induced effect of GATA4 on the osteogenic differentiation of hPDLSCs.Conclusion:GATA4 induces the osteogenic differentiation ability of hPDLSCs via p38 MAPK signaling pathway,it was suggested that GATA4 plays an important role in hPDLSCs involved in injury repair.

关 键 词：GATA4 人牙周膜干细胞 成骨分化 p38 

分 类 号：R780.2[医药卫生—口腔医学]