Genetic Differentiation of the Forest Crested Lizards,Calotes emma alticristatus Schmidt,1925 and C.emma emma Gray,1845(Squamata:Agamidae)Relating to Climate Zones in Thailand  

作　　者：Weerachai SAIJUNTHA Chairat TANTRAWATPAN Warayutt PILAP Sutthira SEDLAK Khathalee SAKDAKHAM Paradee SRIPIROM Warong SUKSAVATE Watee KONGBUNTAD Wittaya TAWONG 

机构地区：[1]Walai Rukhavej Botanical Research Institute,Biodiversity and Conservation Research Unit,Mahasarakham University,Maha Sarakham 44150,Thailand [2]Division of Cell Biology,Department of Preclinical Sciences,Faculty of Medicine,Thammasat University,Rangsit Campus,Pathumthani 12120,Thailand [3]Department of Forest Biology,Faculty of Forestry,Kasetsart University,Bangkok 10900,Thailand [4]Program in Biotechnology,Faculty of Science,Maejo University,Chiang Mai 50290,Thailand [5]Department of Agricultural Science,Faculty of Agriculture,Natural Resources and Environment,Naresuan University,Phitsanulok 65000,Thailand

出　　处：《Asian Herpetological Research》2020年第1期19-27,共9页亚洲两栖爬行动物研究（英文版）

基　　金：This research was supported by Higher Education Research Promotion(HERP),FY2015 to W.Saijuntha.We would like to thank Dr.Adrian R.Plant for English proofreading.

摘　　要：In Thailand,the forest crested lizard,Calotes emma consists of two subspecies,C.emma alticristatus Schmidt,1925 and C.emma emma Gray,1845.This study was performed to determine the genetic diversity and differentiation of C.emma from 16 different localities throughout Thailand.A total of 116 samples were analyzed using the mitochondrial cytochrome c oxidase subunit 1(CO1).Of these,65 and 51 of C.e.alticristatus and C.e.emma were classified into 23(N1-N23)and 21(S1-S21)haplotypes,respectively.There was no shared haplotype between subspecies or between different populations within each subspecies.These haplotypes were classified into four(north-A to north-D)and three(south-A to south-C)haplogroups of C.e aliristaus and C.e.emma,respectively.Phylogenetic analyses retrieved four lineages(classified asⅠtoⅣ).LineagesⅠandⅡcontained the four haplogroups of C.e.alicristatus,whereas lineageⅡandⅣcontained three haplogroups of C.e.emma.These two subspecies live separately in different climate zones,ie.C.e.alticristatus is found in an equatorial winter dry climate,whereas C.e.emma inha bits areas with an equa torial monsoonal clima te.In Thailand, the forest crested lizard, Calotes emma consists of two subspecies, C. emma alticristatus Schmidt, 1925 and C. emma emma Gray, 1845. This study was performed to determine the genetic diversity and differentiation of C. emma from 16 different localities throughout Thailand. A total of 116 samples were analyzed using the mitochondrial cytochrome c oxidase subunit 1(CO1). Of these, 65 and 51 of C. e. alticristatus and C. e. emma were classified into 23(N1 – N23) and 21(S1 – S21) haplotypes, respectively. There was no shared haplotype between subspecies or between different populations within each subspecies. These haplotypes were classified into four(north-A to north-D) and three(south-A to south-C) haplogroups of C. e. alticristatus and C. e. emma, respectively. Phylogenetic analyses retrieved four lineages(classified as I to IV). Lineages I and II contained the four haplogroups of C. e. alticristatus,whereas lineage III and IV contained three haplogroups of C. e. emma. These two subspecies live separately in different climate zones, i.e. C. e. alticristatus is found in an equatorial winter dry climate, whereas C. e. emma inhabits areas with an equatorial monsoonal climate.

关 键 词：Agamid REPTILE Genetic diversity Phylogeny CO1 HAPLOTYPE 

分 类 号：Q953[生物学—动物学]