间歇性θ爆发式磁刺激对高血压大鼠胼胝体髓鞘及局部炎症反应的影响  

Intermittent theta burst stimulation reduced myelin loss,proliferation of astrocytes and activation of microglia in corpus callosum of hypertensive rats

作　　者：蔡颖 邱宝山 廖梦诗 刘晓露 林晶[1] 蓝琳芳[1] 徐光青 范玉华[1] CAI Ying;QIU Baoshan;LIAO Mengshi;LIU Xiaolu;LIN Jing;LAN Linfang;XU Guangqing;FAN Yuhua(Department of Neurology,National Key Clinical Department and Key Discipline of Neurology,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学附属第一医院神经科,广东省重大神经疾病诊治研究重点实验室,国家临床重点专科和国家重点学科,广州510080 [2]首都医科大学北京天坛医院康复医学科 [3]国家神经系统疾病临床医学研究中心

出　　处：《中国神经精神疾病杂志》2020年第3期129-134,共6页Chinese Journal of Nervous and Mental Diseases

基　　金：国家重点研发计划“慢性脑小血管病发病机制及临床诊治新策略研究”(编号:2016YFC1300600);华南神经疾病早期干预及功能修复研究国际合作基地(编号:2015B050501003);广东省神经系统重大疾病诊治工程技术研究中心;广东省神经系统重大疾病诊治转化医学创新平台;广东省神经系统疾病临床医学研究中心。

摘　　要：目的探究间歇性θ爆发式磁刺激对慢性高血压大鼠胼胝体区域髓鞘脱失、星形胶质细胞增生和小胶质细胞活化的改善作用。方法对雄性Sprague-Dawley大鼠随机进行双肾双夹术,制作易卒中型肾血管性高血压大鼠模型。术后22周,模型制备成功的高血压大鼠随机接受连续14 d间歇性θ爆发式磁刺激治疗(intermittent theta burst stimulation,iTBS)(高血压iTBS组,n=6)或假性刺激(高血压假刺激组,n=6),假手术大鼠接受假性刺激(假手术假刺激组,n=6)。HE染色观察胼胝体小动脉形态。免疫荧光染色观察胼胝体MBP标记的髓鞘脱失情况。免疫荧光染色观察GFAP标记的星形胶质细胞数量和IBa-1标记的小胶质细胞数量和形态,以评价星形胶质细胞增生和小胶质细胞活化。结果高血压iTBS组和高血压假刺激组出现明显小动脉管壁增厚。与假手术假刺激组相比,高血压假刺激组胼胝体MBP阳性面积比例减少(P<0.01),GFAP阳性细胞和IBa-1阳性细胞数量明显增加(P<0.01),IBa-1阳性细胞胞体增大,突起变粗,分枝变少。iTBS治疗明显增加了高血压大鼠胼胝体MBP阳性面积比例,降低GFAP阳性细胞和IBa-1阳性细胞数量(P<0.01),IBa-1阳性细胞胞体变小,突起变细,分枝变多。结论iTBS治疗可减轻高血压大鼠胼胝体区域髓鞘脱失,抑制星形胶质细胞增生和小胶质细胞活化。Objective To investigate the effects of intermittent theta burst stimulation(iTBS)on myelin loss,proliferation of astrocytes and activation of microglia in corpus callosum of chronic hypertensive rats.Methods Male Sprague-Dawley rats were randomly received two-kidney,two-clip procedure to establish stroke-prone renovascular hypertensive rats model or sham-operated procedure.At postoperative week 22,hypertensive rats randomly received iTBS treatment(n=6)or sham stimulation(n=6)for 14 consecutive days.Sham-operated rats also received sham stimulation(n=6).HE staining was used to observe small arteries pathologies.Immunofluorescence of MBP was used to evaluate myelin loss.The number GFAP-labeled astrocytes were observed to evaluate the proliferation of astrocytes.The number and morphology of IBa-1-labled microglia were observed to evaluate the activation of microglia.Results Wall thickness of small arteries was increased in hypertensive rats.Compared to sham-operated rats,hypertensive rats with sham stimulation showed a significant decrease in MBP(+)area fraction and a remarkable increase in the numbers of GFAP(+)cells and IBa-1(+)cells(P<0.01).Soma of IBa-1(+)cells were larger and number of branches were decreased.iTBS treatment significantly increased MBP(+)area fraction,and decreased the numbers of GFAP(+)cells and IBa-1(+)cells(P<0.01).IBa-1(+)cells had smaller soma and thinner branches.Conclusion iTBS reduced the myelin loss in corpus callosum of hypertensive rats,and suppressed the proliferation of astrocytes and activation of microglia.

关 键 词：脑白质病变 间歇性θ爆发式磁刺激 髓鞘 星形胶质细胞 小胶质细胞 

分 类 号：R741[医药卫生—神经病学与精神病学]