miR-199a-3p在卵巢癌组织中的表达及其对癌细胞增殖转移的影响  

Expression of miR-199a-3p in ovarian cancer and its effects on proliferation and migration of ovarian cancer cells

作　　者：宋洋[1] 任芳[1] SONG Yang;REN Fang(Shengjing Hospital Affiliated to China Medical University,Shenyang 117004,China)

机构地区：[1]中国医科大学附属盛京医院,沈阳117004

出　　处：《山东医药》2020年第13期1-4,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81501235)。

摘　　要：目的探讨miR-199a-3p在卵巢癌组织中表达情况及其对癌细胞增殖转移能力的影响机制。方法培养四株卵巢癌细胞系SK-OV-3、OV-90、CAOV3、HO-8910S,采用RT-qPCR法检测四株卵巢癌细胞中miR-199a-3p的表达情况。选择miR-199a-3p表达较低的卵巢癌细胞,分为对照组、NC mimic转染组、miR-199a-3p mimic组。对照组不加任何试剂,miR-199a-3p mimic组、NC mimic转染组分别加入miR-199a-3p mimic和NC mimic转染试剂。采用MTT法、Transwell小室试验检测卵巢癌细胞的增殖及迁移能力。RT-qPCR检测过表达miR-199a-3p后卵巢癌细胞中smad1的表达情况,双荧光素酶试验观察miR-199a-3p对smad1的调控作用。结果miR-199a-3p在卵巢癌细胞株CaOV3及OV-90中的表达水平低于其他细胞(P均<0.05)。CaOV3及OV-90细胞过表达miR-199a-3p后,miR-199a-3p mimic组细胞增殖及迁移能力低于NC mimic组(P均<0.05)。miR-199a-3p mimic组细胞中smad1的表达低于NC mimic组(P<0.05)。miR-199a-3p互补结合smad1mRNA的3′UTR区;与wt-smad13′UTR+NC mimic组相比,wt-smad13′UTR+miR-199a-3p mimic组细胞的相对荧光素酶活性降低(P均<0.05)。结论过表达miR-199a-3p能够抑制卵巢癌细胞的增殖和迁移能力,其机制可能与调节smad1的基因表达有关。Objective To investigate the expression of miR-199a-3p in the ovarian cancer tissues and its effects on the proliferation and migration of ovarian cancer cells.Methods Four human ovarian cancer cell lines SK-OV-3,OV-90,CAOV3,and HO-8910S were used in this study,and the expression of miR-199a-3p in four ovarian cancer cell lines were detected by quantitative real-time PCR(RT-qPCR).CaOV3 and OV-90 cells with low expression of miR-199a-3p were divided into the control group,NC mimic transfection group,and miR-199a-3p mimic group.We did not add any reagents to the control group.The cells in the miR-199a-3p mimic group and the NC mimic transfection group were added with miR-199a-3p mimic and NC mimic transfection reagents,respectively.The effects of miR-199a-3p on the proliferation and migration of ovarian cancer cells were determined by MTT and Transwell assays.The expression of smad1 in miR-199a-3p overexpressing cells was detected by RT-qPCR.Dual-luciferase reporter assay was used to determine whether miR-199a-3p regulated the expression of smad1 in the ovarian cancer cells.Results The CaOV3 and OV-90 cells expressed low levels of miR-199a-3p compared with the other two cells(P<0.05).After overexpression of miR-199a-3p in CaOV3 and OV-90 cells,the cell proliferation and migration abilities of the miR-199a-3p mimic group were lower than those of the NC mimic group(both P<0.05).The expression of smad1 in the miR-199a-3p mimic group was lower than that in the NC mimic group(P<0.05).Dual-luciferase reporter assay results demonstrated that miR-199a-3p complementarily bound to the 3′UTR region of smad1mRNA.Compared with the wt-smad13′UTR+NC mimic group,the relative luciferase activity of cells in the wt-smad13′UTR+miR-199a-3p mimic group was reduced(P<0.05).Conclusion Over-expression of miR-199a-3p may inhibit the proliferation and migration of ovarian cancer cells by regulating the expression of smad1 gene.

关 键 词：卵巢肿瘤 细胞增殖 细胞迁移 miR-199a-3p SMAD1 

分 类 号：R737.31[医药卫生—肿瘤]