枯草芽孢杆菌表达与调控工具相关研究进展  

Advances in the Development of Expression and Regulation Systems for Bacillus subtilis

作　　者：张维娇 金学荣 徐雅晴 李江华[1] 堵国成[1] 康振[1] ZHANG Wei-jiao;JIN Xue-rong;XU Ya-qing;LI Jiang-hua;DU Guo-cheng;KANG Zhen(Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Bioengineering,Jiangnan University,Wuxi 214122)

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122

出　　处：《生物技术通报》2020年第4期26-33,共8页Biotechnology Bulletin

基　　金：国家自然基金面上项目(31970085,31670092);国家轻工技术与工程一流学科自主课题(LITE2018-16);江苏省科技支撑项目(BE2019630)。

摘　　要：枯草芽孢杆菌作为革兰氏阳性模式微生物,由于其清晰的遗传背景、高效的分泌能力以及简单的培养条件等优势被广泛的应用于生物技术产业。近年来,随着代谢工程与合成生物学的发展,枯草芽孢杆菌相关表达系统与调控工具研究也取得了很大进展。围绕枯草芽孢杆菌动态调控工具的研究进展,分别从转录水平调控和转录后水平调控两个层面上进行综述,并对调控元件在生物技术中的应用进行了讨论。最后,对未来枯草芽孢杆菌表达与调控工具的发展进行了展望。Bacillus subtilis,as the model Gram-positive bacterium,has been widely used in biotechnology industries because of its clear genetic background,efficient secretion capacity and simple demand of culture conditions.In recent years,with the development of metabolic engineering and synthetic biology,the great progresses on the expression and regulatory systems of B.subtilis have been achieved.Here we reviewed the progress of B.subtilis dynamic regulation tools at transcriptional and post-transcriptional levels.Then,we discussed the application of regulatory elements in biotechnology industries.Meanwhile,we also prospected the research direction of B.subtilis expression and regulatory tools in near future.

关 键 词：枯草芽孢杆菌 转录水平调控 转录后水平调控 动态调控 调控元件 

分 类 号：Q78[生物学—分子生物学]