正确认识新型冠状病毒核酸检测的影响因素  

Understanding the influence factors in viral nucleic acid test of 2019 novel coronavirus

作　　者：莫茜[1] 秦炜 傅启华[1] 关明[3] Mo Xi;Qin Wei;Fu Qihua;Guan Ming(National Children′s Medical Center-Shanghai Children′s Medical Center,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China;Shanghai Rundarongjia Biological Ltd.,Shanghai 201700,China;Department of Laboratory Medicine,Huashan Hospital,Fudan University,Shanghai 200040,China)

机构地区：[1]国家儿童医学中心,上海交通大学医学院附属上海儿童医学中心精准医疗实验诊断中心,上海200127 [2]上海润达榕嘉生物科技有限公司,上海201700 [3]复旦大学附属华山医院检验科,上海200040

出　　处：《中华检验医学杂志》2020年第3期213-216,共4页Chinese Journal of Laboratory Medicine

摘　　要：当前新型冠状病毒的防控已进入关键时期,而基于荧光定量PCR(qRT-PCR)方法的病毒核酸检测作为确诊的重要手段在临床应用中遇到了一些质疑。本文从当前疫情的防控现状、检测技术的开发、疾病本身的特点、以及临床采样、运输等方面进行分析,对可能影响病毒核酸检测性能的因素进行了探讨,并建议了一些可以有效提高病毒核酸检测性能的手段。At present,the prevention and control of new coronavirus has entered a critical period.However,the use of quantitative real-time PCR(qRT-PCR)assays for the detection of viral nucleic acid,as a crucial diagnostic approach,has been doubted in clinical practice.Herein,we have reviewed the current status of epidemic prevention and control,latest development of detection technologies,disease characteristics,clinical sampling and transport.It has also discussed the factors that may affect the performance of viral nucleic acid detection,and suggested some effective methods to improve the detection performance of the assays.

关 键 词：新型冠状病毒肺炎 RNA病毒 分子诊断技术 影响因素分析 核酸检测 

分 类 号：R4[医药卫生—临床医学] R5