螺旋藻多糖提高小白菜抗盐能力  

Improvement of polysaccharide from Spirulina platensis to salt stress resistance of pakchoi

作　　者：程宇娇 刘哲 臧敦秀 马浩天 季春丽[1] 张春辉 岳爱琴[1] 李润植[1] 崔红利 CHENG Yujiao;LIU Zhe;ZANG Dunxiu;MA Haotian;JI Chunli;ZHANG Chunhui;YUE Aiqin;LIRunzhi;CUI Hongli(Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University,Taigu 030801,Shanxi,China)

机构地区：[1]山西农业大学分子农业与生物能源研究所,山西太谷030801

出　　处：《中国瓜菜》2020年第4期43-49,共7页China Cucurbits And Vegetables

基　　金：国家自然科学基金(31902394);山西省优秀博士来晋工作奖励资金科研项目(SXYBKY2019036);山西省重点研发项目(201803D31063);山西农谷建设科研专项(SXNGJSKYZX201906);山西省优秀研究生创新项目(2019SY226);山西省煤基重大科技专项项目(FT-2014-01);山西省重点研发重点项目(201603D312005);农业部“948”项目(2014-Z39);晋中市重点研发项目(Y192012)。

摘　　要：在水培条件下探究不同浓度的PSP(螺旋藻多糖)能否提高‘奶油四季小白菜’的抗盐性。生理生化检测显示,NaCl(5 g·L-1)胁迫显著抑制小白菜根长和鲜质量,且造成严重的生理伤害。水培液中添加PSP使得小白菜根长、鲜质量和叶绿素含量增加。PSP处理提高了小白菜体内超氧化物岐化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,增加了抗坏血酸、类胡萝卜素和脯氨酸含量。然而,PSP处理明显降低了小白菜体内MDA和H2O2含量。研究结果表明,PSP可通过提高盐胁迫下小白菜抗氧化酶活性、非酶类抗氧化物质含量以及积累渗透调节物质,有效清除过量的活性氧和缓解氧化损伤,进而增强小白菜抗盐胁迫的能力。作为一种绿色的植物生长促进剂,PSP可用于高值蔬菜的有机生产。In this study, polysaccharide from Spirulina platensis(PSP)was used as a biostimulant. The effect of PSP with different concentration on salt resistance of pakchoi(Brassica chinensis L.)under hydroponics was investigated.Physiological and biochemical analysis showed that NaCl stress(5 g·L-1)significantly inhibited the root length and fresh weight of pakchoi plants and caused severe physiological damage. Adding different dosages of PSP in hydroponic solution resulted in the enhancement in root length, fresh weight, and chlorophyll content of pakchoi. Moreover, PSP treatments increased the activities of superoxide dismutase(SOD), peroxidase(POD)and catalase(CAT)in pakchoi,together with the elevated levels in the contents of ascorbic acid, carotenoid and proline, but reduction in MDA and H2O2.These results indicated that PSP could increase antioxidant enzyme activities, the content of non-enzyme antioxidants,and the accumulation of osmotic adjustment substances, and consequently result in the effective remove of excess reactive oxygen species and alleviation of oxidative damages caused by salt stress, leading to improvement in salt stress resistance of pakchoi plants. As a green growth-promoting regulator, PSP has potential application in the organic cultivation of high-value vegetables.

关 键 词：小白菜 螺旋藻多糖 NACL胁迫 生理特性 抗氧化性 

分 类 号：S634.3[农业科学—蔬菜学]