烟碱型乙酰胆碱受体在面神经支配的口轮匝肌和躯体神经支配的腓肠肌中的表达差异研究  

A Study on the Different Expression of Nicotinic Acetylcholine Receptors in the Facial Nerve Innervated Orbicularis Oris and the Somatic Nerve Innervated Gastrocnemius

作　　者：吴爽 黄勇 邢怡安 陈莲华[1] 李士通[1] WU Shuang;HUANG Yong;XING Yi-an;CHEN Lian-hua;LI Shi-tong(Department of anesthesiology,Shanghai general hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,201600,China)

机构地区：[1]上海交通大学附属第一人民医院麻醉科,上海201600

出　　处：《现代生物医学进展》2020年第2期209-213,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81271075);国家自然科学基金项目(81870714)。

摘　　要：目的:研究烟碱型乙酰胆碱受体在在面神经支配的口轮匝肌和躯体神经支配的腓肠肌运动终板处的表达差异及可能的原因。方法:分离SD大鼠的口轮匝肌和腓肠肌,通过免疫共沉淀技术计算口轮匝肌和腓肠肌肌肉特异性激酶(Muscle Specific Kinase,MuSK)的表达以及MuSK磷酸化水平。对MuSK的上游信号通路中能使其发生磷酸化的集聚蛋白Agrin、低密度脂蛋白受体相关蛋白4(low-density lipoprotein receptor-related protein 4,Lrp4)以及表皮生长因子家族受体ErbB2、ErbB3和ErbB4(epidermal growth factor receptor)免疫荧光染色,计算这两条不同通路中的蛋白在运动终板处的表达水平。结果:口轮匝肌中MuSK磷酸化水平显著高于腓肠肌(P<0.05)。口轮匝肌与腓肠肌的运动终板处的Agrin和Lrp4表达没有显著差异(P>0.05)。口轮匝肌ErbB2、ErbB3、ErbB4的表达显著高于其在腓肠肌运动终板处的表达(P<0.01)。结论:口轮匝肌和腓肠肌运动终板ErbB、ErbB3、ErbB4的差异表达造成MuSK磷酸化水平不同,可能是两种肌肉运动终板处烟碱型乙酰胆碱亚基表达量不同的原因。Objective:To study the different expression of nicotinic acetylcholine receptors in the orbicularis oris and gastrocnemius.Methods:Orbicularis oris and gastrocnemius were harvested from rats.The percentage of phosphorylated MuSK(Muscle Specific Kinase)was figured out by immunoprecipitation.Immunofluorescence was used to measure the expressions of Agrin,Lrp4(low-density lipoprotein receptor-related protein 4)and epidermal growth factor receptor ErbB2,ErbB3,ErbB4,which can activate the phosphorylation of MuSK.Results:The percentage of phosphorylated MuSK in the orbicularis oris was higher than in the gastrocnemius(P<0.05).The expressions of Agrin,Lrp4 showed no difference in the orbicularis oris and gastrocnemius(P>0.05).ErbB2,ErbB3 and ErbB4 expressed differently in the orbicularis oris and gastrocnemius(P<0.01).Conclusion:The different expressions of ErbB2,ErbB3,ErbB4 result in different level of phosphorylated MuSK,which could contribute to the different expression of nicotinic acetylcholine receptors in the facial nerve innervated orbicularis oris and somatic nerve innervated gastrocnemius.

关 键 词：诱发肌电位 非去极化肌松药 肌肉特异性激酶 表皮生长因子受体 

分 类 号：R-33[医药卫生] Q429.7[生物学—神经生物学]