牙本质基质蛋白1糖基化修饰对小鼠骨骼肌损伤修复的影响  

Effects of glycosylation of dentin matrix protein 1 on repair of mouse skeletal muscle injury

作　　者：周梦琪 范琪琪 孙瑶 ZHOU Meng-qi;FAN Qi-qi;SUN Yao(Dept.of Implantology,School&Hospital of Stomatology,Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Shanghai 200072,China)

机构地区：[1]同济大学附属口腔医院种植科,上海牙组织修复与再生工程技术研究中心,上海200072

出　　处：《同济大学学报（医学版）》2020年第2期198-204,共7页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金(81771043)。

摘　　要：目的探讨牙本质基质蛋白1糖基化(DMP1-PG)在小鼠骨骼肌损伤修复中的作用及其机制。方法选取5周龄(年轻)和12月龄(老龄)野生型小鼠,通过免疫荧光染色和RT-qPCR确定DMP1-PG在小鼠骨骼肌中的表达。通过建立5周龄DMP1-S89G基因突变小鼠和野生型小鼠骨骼肌冰冻损伤模型,损伤后2、7、14d取损伤处骨骼肌,H-E染色观察骨骼肌愈合过程。通过RT-qPCR检测骨骼肌中损伤修复相关mRNA的表达变化。结果 DMP1-PG在年轻小鼠骨骼肌肌纤维连接处大量表达,在老龄小鼠骨骼肌中表达显著下降(P<0.01)。在年轻小鼠腓肠肌损伤修复过程中DMP1-PG表达上调(P<0.01),DMP1-PG缺失后,骨骼肌修复功能减弱,肌再生相关因子和血管再生因子表达减少(P<0.01),小鼠腓肠肌损伤修复受到抑制。结论牙本质基质蛋白1糖基化修饰能够通过调节肌再生相关因子和血管再生因子的表达,影响年轻小鼠骨骼肌损伤修复。Objective To investigate the role and mechanism of glycosylation of dentin matrix protein 1(DMP1-PG) in mouse skeletal muscle injury repair. Methods The expression of DMP1-PG in skeletal muscle of young(5 weeks)and old(12 months) wild-type(WT) mice was determined by immunofluorescence staining and RT-qPCR, respectively. The skeletal muscle frozen injury was induced in gastrocnemius of 5-week-old DMP1-S89 G mutant mice(with reduced glycosylation of DMP1) and WT mice, and the samples of injured skeletal muscle were taken at 2, 7 and 14 day after injury. The healing process of skeletal muscle was observed by H-E staining, the expression of regenerating factor mRNA in skeletal muscle was detected by RT-qPCR. Results DMP1-PG was abundantly expressed at the junction of young mouse skeletal muscle fibers and significantly decreased in aged mouse skeletal muscle(P<0.01). During the repair of gastrocnemius injury in young mice, DMP1-PG expression was up-regulated(P<0.01). After DMP1-PG deletion, mouse skeletal muscle repair function was weakened, the expression of muscle regeneration related factors and angiogenesis factors was decreased(P<0.01), and gastrocnemius repair was inhibited. Conclusion The glycosylation of DMP1 can affect the repair of skeletal muscle injury in young mice by regulating the expression of muscle regeneration related factors and angiogenesis factors.

关 键 词：牙本质基质蛋白1糖基化 冰冻损伤 骨骼肌 损伤修复 再生因子 

分 类 号：R781[医药卫生—口腔医学]