人骨髓间充质干细胞对舌鳞癌细胞系Cal-27侵袭作用的影响  

Effect of human bone marrow mesenchymal stem cells on invasion of tongue squamous cell carcinoma cell line Cal-27

作　　者：徐竹青 张馨予[1] 王少如 李涛[2] 董刚[2] 李凤梅[2] 郑建金[2] 王云英[4] 徐晓娜[4] XU Zhu-qing;ZHANG Xin-yu;WANG Shao-ru;LI Tao;DONG Gang;LI Feng-mei;ZHENG Jian-jin;WANG Yun-ying;XU Xiao-na(School of Stomatology,Qingdao University.Qingdao 266000,Shandong Province;Qingdao City Hospital Stomatological Medical Center.Qingdao 266000,Shandong Province;Dalian Medical University.Dalian 116044,Liaoning Province;Basic Laboratory of Qingdao Municipal Hospital.Qingdao 266000,Shandong Province,China)

机构地区：[1]青岛大学口腔医学院,山东青岛266000 [2]青岛市市立医院口腔医学中心,山东青岛266000 [3]大连医科大学,辽宁大连116044 [4]青岛市市立医院基础实验室,山东青岛266000

出　　处：《上海口腔医学》2020年第2期155-161,共7页Shanghai Journal of Stomatology

基　　金：青岛市科技局重点项目(15-9-2-79-nsh)。

摘　　要：目的:探讨人骨髓间充质干细胞(human bone marrow mesenchymal stem cells,hBM-MSCs)对舌鳞癌细胞系Cal-27侵袭的影响及作用机制。方法:分别培养hBM-MSCs和Cal-27,倒置显微镜下观察细胞形态。hBM-MSCs和Cal-27共培养,获得共培养后的Cal-27细胞。划痕实验观察Cal-27的迁移面积;Transwell迁移及侵袭实验观察Cal-27的迁移及侵袭细胞数目,绘制条形图。使用荧光定量PCR技术,观察hBM-MSCs对Cal-27侵袭相关肿瘤标志物E-cadherin、twist、slug、snail、MMP-2、MMP-9基因表达的影响;使用Western免疫印迹技术,观察hBM-MSCs对Cal-27侵袭相关肿瘤标志物MMP-2、MMP-9蛋白表达的影响。采用SPSS 19.0软件包对数据进行统计学分析。结果:hBM-MSCs可促进Cal-27的侵袭,伴随E-cadherin下调,twist、slug、snail、MMP-2、MMP-9基因表达上调及MMP-2、MMP-9蛋白表达上调。结论:hBM-MSCs促进Cal-27细胞的侵袭作用,可能与其上调Cal-27细胞侵袭作用相关肿瘤标志物的表达有关。PURPOSE: To investigate the effect of human bone marrow mesenchymal stem cells(hBM-MSCs) on invasion of tongue squamous cell carcinoma cell line Cal-27 and its mechanism. METHODS: hBM-MSCs and Cal-27 were cultured respectively, and the morphology of the cells was observed under an inverted microscope. The co-cultured Cal-27 cells were obtained by co-culture of hBM-MSCs and Cal-27. The migration area of Cal-27 was observed by scratch test;transwell migration and invasion experiments were performed to observe migration and invasion of Cal-27, and a bar graph was then drawn. Fluorescence quantitative PCR was used to observe the effect of hBM-MSCs on gene expression of the tumor markers E-cadherin, twist, slug, snail, MMP-2 and MMP-9. Western blot was used to observe the effect of hBM-MSCs on protein expression of MMP-2 and MMP-9, related to the invasion of Cal-27. SPSS 19.0 software package was used for statistical analysis of the data. RESULTS: Under the influence of hBM-MSCs, the invasion of Cal-27 was promoted, accompanied by down-regulation of E-cadherin, up-regulation of twist, slug, snail, MMP-2, MMP-9 and upregulation of MMP-2 and MMP-9 expression. CONCLUSIONS: hBM-MSCs can promote invasion of Cal-27 cells, which may be related to up-regulation of the expression of tumor markers related to invasion of Cal-27 cells.

关 键 词：人骨髓间充质干细胞 舌鳞癌细胞 侵袭 肿瘤标志物 

分 类 号：R739.86[医药卫生—肿瘤] Q813.11[医药卫生—临床医学]