Inhibition of cell proliferation and migration by Oroxylum indicum extracts on breast cancer cells via Rac1 modulation  

作　　者：Benjaporn Buranrat Sirintra Noiwetch Tippaporn Suksar Aphimook Ta-ut 

机构地区：[1]MD Program,Faculty of Medicine,Mahasarakham University,Muang District,Maha Sarakham,44000,Thailand [2]Applied Thai Traditional Medicine Program,Faculty of Medicine,Mahasarakham University,Muang District,Maha Sarakham,44000,Thailand

出　　处：《Journal of Pharmaceutical Analysis》2020年第2期187-193,共7页药物分析学报（英文版）

基　　金：financially supported by Mahasarakham University(Fast Track 2019)。

摘　　要：In this study,we investigated how Oroxylum indicum leaf and fruit extracts affect the viability and migration of MCF-7 breast cancer cells and the mechanisms of action responsible for these effects.MCF-7 cells treated with the extracts were examined using the sulforhodamine B,colony formation and caspase 3 activity assays,and by Western blotting.O.indicum extracts were found to inhibit MCF-7 cell growth in a concentration-and time-dependent manner,with 48 h IC50 values of 57.02±2.85μg/mL and 131.3±19.2μg/mL for leaf and fruit extracts,respectively.Further,the O.indicum leaf extract caused a reduction in MCF-7 cell viability,induction of MCF-7 cell apoptosis and ROS formation,and an increase in caspase 3 activity.Also,the two extracts inhibited MCF-7 cell migration and reduced both MMP 9 and ICAMP1 gene expression and MMP9 protein expression.Additionally,O.indicum extracts greatly reduced expression of the cell cycle regulatory protein Rac1 in the mevalonate pathway.In summary,O.indicum leaf and fruit extracts reduce breast cancer cell growth,cell viability and cell migration.O.indicum constituents could,therefore,be useful for augmenting the activity of chemotherapeutic drugs employed to treat breast cancer.

关 键 词：Oroxylum indicum Reactive oxygen species(ROS) MIGRATION Cell VIABILITY Matrix metalloproteinase(MMP) Apoptosis 

分 类 号：R737.9[医药卫生—肿瘤]