内皮细胞抑制素和血管抑素在翼状胬肉组织中的表达及临床意义  

Expression and clinical significance of endostatin and angiostatin in pterygium

作　　者：赵静 陈伟伟 金忠平 章峥嵘 Zhao Jing;Chen Weiwei;Jin Zhongping;Zhang Zhengrong(Department of Ophthalmology,the Traditional Chinese Medicine Hospital of Yiwu,Yiwu,Zhejiang 322000,China;Department of Pathology,Fuyuan Private Hospital of Yiwu,Yiwu,Zhejiang 322000,China;Department of Ophthalmology,the First Affiliated Hospital of Jiaxing,Jiaxing,Zhejiang 314502,China)

机构地区：[1]义乌市中医医院眼科,浙江省322000 [2]义乌市復元私立医院病理科,浙江省322000 [3]嘉兴市第一医院眼科,浙江省314502

出　　处：《中国基层医药》2020年第7期791-794,共4页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的:探讨内皮细胞抑制素(ES)和血管抑素(AS)在翼状胬肉组织中的表达及临床意义。方法:选择2016年1月至2018年12月义乌市中医医院眼科手术切除的翼状胬肉组织60例(60眼)和对侧健康人球结膜组织60例(60眼)。苏木精-伊红染色法(HE)染色观察翼状胬肉组织和健康球结膜组织形态学变化。Western blot测量翼状胬肉组和对照组组织中ES和AS蛋白水平。结果:HE染色:健康球结膜组织基质层为结缔组织,上皮层为柱状上皮。翼状胬肉基质层见大量新生血管、成纤维细胞、胶原纤维,血管周围见炎性细胞浸润,上皮呈不同程度增生。翼状胬肉组织中ES和AS蛋白水平[(0.35±0.12)、(0.62±0.17)]均高于对照组[(0.13±0.08)、(0.16±0.09)]( t=11.816、18.524, P=0.000、0.000)。复发组翼状胬肉组织中ES和AS蛋白水平[(0.63±0.15)、(0.87±0.21)]均高于初发组[(0.22±0.11)、(0.45±0.16)]( t=17.073、12.323, P=0.000、0.000)。翼状胬肉组织中ES与AS呈正相关( r=0.571, P=0.000)。 结论:翼状胬肉组织中ES和AS水平升高,ES和AS可能参与翼状胬肉的发生、复发过程。Objective To investigate the expression and clinical significance of endostatin(ES)and angiostatin(AS)in pterygium.Methods From January 2016 to December 2018,60 cases(60 eyes)of pterygium tissue and 60 cases(60 eyes)of normal human conjunctival tissue were selected from the eye surgery of Traditional Chinese Medicine Hospital of Yiwu.HE staining was used to observe the morphological changes of pterygium and normal conjunctival tissue.Western blot was used to measure ES and AS protein levels in the tissues of pterygium group and control group.Results HE staining showed that in the normal bulbar conjunctival tissue,the stromal layer was connective tissue and the epithelial layer was columnar epithelium;in the pterygium,the basal layer had a large number of new blood vessels,fibroblasts,collagen fibers,and inflammatory cells infiltrated around the blood vessels;the epithelium showed different degrees of hyperplasia.The protein levels of ES and AS in pterygium tissues[(0.35±0.12),(0.62±0.17)]were higher than those in the control group[(0.13±0.08),(0.16±0.09)](t=11.816,18.524,P=0.000,0.000).The protein levels of ES and AS in the pterygium tissues of the recurrent group[(0.63±0.15),(0.87±0.21)]were higher than those in the initial group[(0.22±0.11),(0.45±0.16)](t=17.073,12.323,P=0.000,0.000).There was positive correlation between ES and AS in pterygium(r=0.571,P=0.000).Conclusion The levels of ES and AS in pterygium tissue are increased,and ES and AS may be involved in the occurrence and recurrence of pterygium.

关 键 词：翼状胬肉 抑制素类 血管抑素类 内皮细胞 复发 

分 类 号：R77[医药卫生—眼科]