Epidural Spinal Cord Stimulation Promotes Motor Functional Recovery by Enhancing Oligodendrocyte Survival and Differentiation and by Protecting Myelin after Spinal Cord Injury in Rats  

作　　者：Gang Li Zhong-Kai Fan Guang-Fei Gu Zhi-Qiang Jia Qiang-Qiang Zhang Jun-Yu Dai Shi-Sheng He 

机构地区：[1]Department of Orthopedics,Shanghai Tenth People's Hospital,Tongji University School of Medicine,Shanghai 200072,China [2]Spinal Pain Research Institute,Tongji University School of Medicine,Shanghai 200072,China [3]Department of Orthopaedics,The First Affiliated Hospital,Jinzhou Medical University,Jinzhou 121001,China [4]Department of Spinal Surgery,The Second Affiliated Hospital,Henan University of Science and Technology,Luoyang 471003,China

出　　处：《Neuroscience Bulletin》2020年第4期372-384,共13页神经科学通报（英文版）

基　　金：supported by the Natural Science Foundation of Liaoning Province (201602277);the Science and Technology Planning Project of Liaoning Province (LJQ2014091).

摘　　要：Epidural spinal cord stimulation (ESCS) markedly improves motor and sensory function after spinal cord injury (SCI), but the underlying mechanisms are unclear.Here, we investigated whether ESCS affects oligodendrocyte differentiation and its cellular and molecular mechanisms in rats with SCI. ESCS improved hindlimb motor function at 7 days, 14 days, 21 days, and 28 days after SCI.ESCS also significantly increased the myelinated area at 28days, and reduced the number of apoptotic cells in the spinal white matter at 7 days. SCI decreased the expression of 20,30-cyclic-nucleotide 30-phosphodiesterase (CNPase,an oligodendrocyte marker) at 7 days and that of myelin basic protein at 28 days. ESCS significantly upregulated these markers and increased the percentage of Sox2/CNPase/DAPI-positive cells (newly differentiated oligodendrocytes) at 7 days. Recombinant human bone morphogenetic protein 4 (rh BMP4) markedly downregulated these factors after ESCS. Furthermore, ESCS significantly decreased BMP4 and p-Smad1/5/9 expression after SCI,and rh BMP4 reduced this effect of ESCS. These findings indicate that ESCS enhances the survival and differentiation of oligodendrocytes, protects myelin, and promotes motor functional recovery by inhibiting the BMP4-Smad1/5/9 signaling pathway after SCI.

关 键 词：SPINAL CORD injury EPIDURAL SPINAL CORD stimulation OLIGODENDROCYTE Differentiation REMYELINATION 

分 类 号：R651.2[医药卫生—外科学]