生长分化因子11通过TRAF6和p38通路促进破骨样细胞的增殖和分化  

GDF11 Promotes the Proliferation and Differentiation of Osteoclast-like Cells Through TRAF6 and p38 Pathway

作　　者：李宗鲜 许明珠 吉木伍各木 龚思月 呼海燕[1] Li Zongxian;Xu Mingzhu;Ji Muwugemu;Gong Siyue;Hu Haiyan(Chengdu Medical College, Chengdu 610500, China;Sichuan Nursing Vocational College, Chengdu 610100, China)

机构地区：[1]成都医学院,成都610500 [2]四川护理职业学院,成都610100

出　　处：《成都医学院学报》2020年第2期196-199,237,共5页Journal of Chengdu Medical College

基　　金：四川省教育厅自然科学基金项目(No:17ZA0115);国家大学生创新创业训练计划项目(No:201813705035,No:201813705034)。

摘　　要：目的观察生长分化因子11(GDF11)对RAW264.7细胞增殖、分化及破骨细胞相关转录因子RANK、肿瘤坏死因子相关受体因子(tumor necrosis factor receptor-associated factors,TRAF6)和p38 mRNA的表达。方法培养RAW264.7细胞,通过CCK-8法、TRAP染色、q-PCR技术及骨吸收实验测定细胞增殖及RANK、TRAF6和p38 mRNA表达。结果体积浓度为0.1、1、2 ng/mL的GDF11促进细胞增殖;GDF11促进RANK、TRAF6和p38 mRNA表达;GDF11、GDF11+RANKL和RANKL使TRAP活性明显增加;GDF11、GDF11+RANKL和RANKL均使破骨样细胞的骨吸收能力增强。结论GDF11可能通过增加RANK、TRAF6和p38 mRNA表达,促进破骨样细胞增殖和提高TRAP活性,并促进破骨细胞的骨吸收作用。Objective To investigate the effect of GDF11 on the proliferation and differentiation of RAW264.7 cells and the expression of RANK,TRAF6 and p38 mRNA.Methods The RAW264.7 cells were cultured and their proliferation and differentiation and the expression of RANK,TRAF6 and p38 mRNA were determined by CCK-8,TRAP staining,q-PCR technique and bone resorption experiment.Results GDF11 promoted the proliferation and differentiation of cells at the concentration of 0.1,1 and 2 ng/mL,respectively,and it significantly increased the expression of RANK,TRAF6 and p38 mRNA.The TRAP activity was significantly increased by GDF11,GDF11+RANKL and RANKL,respectively.The bone resorption capacity was increased by GDF11,GDF11+RANKL and RANKL,respectively.Conclusion GDF11 may promote the proliferation and bone resorption of osteoclast-like cells and increase the TRAP activity by promoting the expression of RANK,TRAF6 and p38 mRNA.

关 键 词：RAW264.7细胞 GDF11 RANKL 信号通路 骨吸收 

分 类 号：R363[医药卫生—病理学]