高效毛细管电泳法同时分离检测六种地龙多肽  

Simultaneous Separation and Detection of Six Earthworm Peptides by Efficient Capillary Zone Electrophoresis

作　　者：周鑫悦 张诗琪 林露 陈莉[1] 李琦[1] 赖昕[1] 余丽双[1] Zhou Xinyue;Zhang Shiqi;Lin Lu;Chen Li;Li Qi;Lai Xin;Yu Lishuang(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福州350122

出　　处：《世界科学技术-中医药现代化》2019年第12期2569-2575,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：福建省科技厅面上项目(2018J01871):基于多模式毛细管电泳及其联用技术的动物类中药质量控制新方法研究,负责人:余丽双;福建省科技厅重点项目(2017Y0051):基于抗肝癌活性导向的闽产中成药消癥益肝片制剂工艺改进研究,负责人:赖昕;福建省科技厅面上项目(2017J01839):基于脂质体毛细管电泳技术的太子参活性成分快速筛选新方法研究,负责人:李琦。

摘　　要：地龙药用历史悠久,但目前未见对其活性多肽进行分离检测的报道。本文建立了一种同时分离检测六种地龙多肽的毛细管区带电泳新方法。实验发现,在以60 mmol·L^-1乙酸铵-50 mmol·L^-1氢氧化钠(pH=8.0)为运行缓冲液,以20 kV为分离电压的分析条件下,六种地龙多肽可在10 min内到达基线分离。经方法学验证,六种多肽精密度良好,检测限(S/N=3)可低至为1.0μg·mL^-1。Earthworms have been used as a traditional medicine in China for thousands of years. However, there have not been publications related to the analysis of their active peptide. The experiment found that by using 60 mmol·L^-1 ammonium acetate-50 mmol·L^-1 sodium hydroxide(pH = 8.0) as running buffer and 20 kV as separation voltage,the six kinds of earthworm peptides reached baseline separation within 10 min. After the methodological verification, good precision was obtained, and the detection limit(S/N = 3) could be as low as 1.0 μg·mL^-1.

关 键 词：地龙 多肽 毛细管电泳 

分 类 号：R33[医药卫生—人体生理学]