Acetylated HOXB9 at lysine 27 is of differential diagnostic value in patients with pancreatic ductal adenocarcinoma  

作　　者：Xiaoran Sun Jiagui Song Jing Zhang Jun Zhan Weigang Fang Hongquan Zhang 

机构地区：[1]Department of Human Anatomy,Histology and Embryology,Key Laboratory of Carcinogenesis and Translational Research,Ministry of Education,and State Key Laboratory of Natural and Biomimetic Drugs,Peking University Health Science Center,Beijing 100191,China [2]Department of Pathology,Peking University Health Science Center,Beijing 100191,China

出　　处：《Frontiers of Medicine》2020年第1期91-100,共10页医学前沿（英文版）

基　　金：supported by grants from the Ministry of Science and Technology of China(Nos.2016YFC1302103 and 2015CB553906);the National Natural Science Foundation of China(Nos.81730071,81230051,81472734,and 31170711);the Beijing Natural Science Foundation(Nos.7120002 and 7171005);This work was also supported by a grant from the National Natural Science Foundation of China(No.81773199)to Jun Zhan.

摘　　要：Pancreatic ductal adenocarcinoma(PDAC)is the ninth most common human malignancy and the sixth leading cause of cancer-related death in China.AcK27-HOXB9 is a newly identified HOXB9 post-transcriptional modification that can predict the outcome in lung adenocarcinoma and colon cancer well.However,the role of AcK27-HOXB9 in PDAC is unclear.The present study aims to investigate the differential diagnostic role of patients with AcK27-HOXB9 PDAC.Tissue microarrays consisting of 162 pancreatic tumor tissue samples from patients with PDAC and paired normal subjects were used to examine HOXB9 and AcK27-HOXB9 levels and localizations by immunohistochemical analysis and Western blot assay,respectively.HOXB9 was upregulated(P<0.0001),and AcK27-HOXB9(P=0.0023)was downregulated in patients with PDAC.HOXB9 promoted(P=0.0115),while AcK27-HOXB9(P=0.0279)inhibited PDAC progression.AcK27-HOXB9 predicted favorable outcome in patients with PDAC(P=0.0412).AcK27-HOXB9 also suppressed PDAC cell migration in a cell migration assay.The results of this study showed that HOXB9 promoted and AcK27-HOXB9 suppressed PDAC progression.The determination of ratio between HOXB9 and AcK27-HOXB9 exhibited potential diagnostic value in patients with PDAC.

关 键 词：HOXB9 AcK27-HOXB9 PDAC 

分 类 号：R735.9[医药卫生—肿瘤]